Pirin Polypeptide Modulates NF-kB to Restore Intestinal Barrier
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Solution Overview
Problem
Current treatments for inflammatory and autoimmune disorders, particularly those affecting the gastrointestinal tract, often fail to effectively reduce inflammation and maintain intestinal integrity, leading to ongoing tissue damage and disruption.
Innovation Solution
The use of a polypeptide HP, derived from Bacteroides thetaiotaomicron, which modulates inflammation by regulating NF-κB pathways and improving intestinal barrier function, is proposed, along with its encoding polynucleotide sequence and expression vectors for therapeutic and nutritional applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current treatments for inflammatory and autoimmune disorders are used, then some inflammatory symptoms may be temporarily managed, but they fail to effectively reduce inflammation and maintain intestinal integrity, leading to ongoing tissue damage and disruption
Solution Approach 1:
The patent extracts and utilizes a specific protein (Pirin) from the natural source (Bacteroides thetaiotaomicron bacterium) that has the desired anti-inflammatory effect. This extracted protein is then applied therapeutically to achieve reliable inflammation reduction and intestinal integrity maintenance without the harmful side effects of current treatments
Solution Approach 2:
The Pirin protein acts as a natural intermediary substance that mediates the anti-inflammatory effect by regulating NF-κB pathways. This natural mediator provides effective inflammation reduction while maintaining intestinal integrity, resolving the contradiction between treatment effectiveness and tissue protection
2Object-affected harmful factors
If conventional anti-inflammatory treatments are administered, then inflammation may be partially controlled, but intestinal barrier function deteriorates and tissue damage progresses
Solution Approach 1:
The patent converts the natural anti-inflammatory mechanism of the Bacteroides thetaiotaomicron bacterium into a therapeutic benefit. The Pirin protein, naturally produced by this bacterium, is extracted and used to treat inflammation while simultaneously protecting intestinal barrier function, thus converting a natural biological mechanism into a beneficial therapeutic agent that addresses both inflammation control and tissue protection
3Object-affected harmful factors
If current therapeutic approaches are used, then some inflammatory symptoms are addressed, but pro-inflammatory pathways remain activated and gut microbiota composition is not improved
Solution Approach 1:
The Pirin protein exerts its anti-inflammatory effect by preemptively regulating NF-κB pathways before pro-inflammatory gene expression is fully activated. This preliminary action prevents the activation of harmful pro-inflammatory pathways while reducing inflammatory symptoms, addressing both aspects simultaneously rather than reacting after damage occurs
Data Source
AI summary
The present invention relates to polypeptide HP, or a polynucleotide sequence encoding polypeptide HP, or a host cell comprising said polynucleotide sequence, or a host cell comprising an expression vector comprising said polynucleotide sequence, for use in the treatment and/or prevention of a disorder in a subject; wherein said disorder is an inflammatory disorder and/or an autoimmune disorder; wherein said polypeptide has at least 75% identity to SEQ ID NO 2, SEQ ID NO 4 or SEQ ID NO 6 or variants, homologues, fragments or derivatives thereof; and wherein said polynucleotide sequence encodes a polypeptide which has at least 75% identity to SEQ ID NO 2, SEQ ID NO 4 or SEQ ID NO 6 or variants, homologues, fragments or derivatives thereof and/or wherein said polynucleotide sequence has at least 75% identity to SEQ ID NO 1, SEQ ID NO 3 or SEQ ID NO 5 or variants, homologues, fragments or derivatives thereof.