Progenitor Cell Isolation via Placental Tissue Extract
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Solution Overview
Problem
Current methods for isolating progenitor cells from fetal tissues are inadequate and inefficient, often damaging the extracellular matrix and destroying vital regulatory proteins, which compromises the cells' proliferative and differentiation capabilities.
Innovation Solution
A novel method utilizing the natural collagenolytic, fibrinolytic, and proteolytic activity of abortive placenta tissue to isolate progenitor cells from fetal organs with minimal manipulation, employing a three-open loops in situ organs perfusion system, ensuring the cells' viability and integrity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If collagenase and DNase are used to destroy extracellular matrix for progenitor cell isolation, then cell separation is achieved, but transmembrane regulatory proteins are destroyed and cell viability is compromised
Solution Approach 1:
The patent changes the chemical parameters of the extracellular matrix degradation process by using fetal placental tissue extract instead of harsh chemical enzymes. This natural extract contains proteolytic and fibrinolytic activities that selectively degrade the fetal extracellular matrix while preserving the integrity of progenitor cells and their regulatory proteins, thus resolving the contradiction between isolation efficiency and cell viability
Solution Approach 2:
The patent introduces an intermediary substance - fetal placental tissue extract - that mediates the separation process. This extract acts as a gentle proteolytic and fibrinolytic agent that breaks down the extracellular matrix without directly contacting and damaging the progenitor cells, thereby achieving both effective separation and preservation of cell function
2Productivity
If extensive ECM remodeling is performed to isolate cells, then separation is achieved, but proliferative and differentiation capacity of cells is negatively altered
Solution Approach 1:
The patent modifies the remodeling parameters by using physiological concentrations of natural proteolytic and fibrinolytic enzymes from fetal placenta. This controlled, gentle approach selectively degrades matrix components while maintaining the biochemical environment necessary for preserving cell proliferative and differentiation capacity
Solution Approach 2:
The patent converts the naturally occurring proteolytic and fibrinolytic activities of fetal placenta - which would otherwise be waste material - into a beneficial tool for gentle matrix remodeling. This approach achieves effective cell separation while the natural origin of these enzymes ensures compatibility with cell function preservation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively isolates a high number of viable progenitor cells from various fetal organs with enhanced proteolytic and fibrinolytic activity, demonstrating safety and efficacy without toxic or side effects, suitable for therapeutic use.
Implementation Method 1
used a soft natural proteolytic, collagenolytic and fibrinolytic activity of abortive placenta
Implementation Method 2
used a soft natural proteolytic, collagenolytic and fibrinolytic activity of abortive placenta
Implementation Method 3
used a soft natural proteolytic, collagenolytic and fibrinolytic activity of abortive placenta
Data Source
AI summary
The present invention is directed to novel methods comprising the isolation of viable fetal progenitor cells from organs of aborted fetus. The invention comprises the use of natural proteolytic, collagenolytic and fibrinolytic activity of autologous abortive placenta tissue extract.