Placental Acellular Biologic Composition for Tissue Regeneration
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Solution Overview
Problem
Existing methods for producing safe allogeneic tissue regenerative compositions face challenges in minimizing antigenic properties to prevent inflammation, leading to reduced viable cell harvest and diminished regenerative capacity, particularly when using placental tissues.
Innovation Solution
A biological composition derived from placental tissues, comprising non-whole cellular components such as exosomes, transcriptosomes, and extracellular matrix components, is developed, which is treated with a polyampholyte cryoprotectant to enhance regenerative resonance and pluripotency, allowing for effective tissue regeneration without the need for whole cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If stem cells are isolated and purified from placental tissues, then regenerative capacity is maintained, but viable cell harvest is greatly diminished
Solution Approach 1:
The patent extracts and removes whole cells from placental tissues, retaining only non-cellular components including extracellular matrix, growth factors, and signaling molecules. This extraction approach eliminates the need for cell-based therapy while preserving regenerative properties through the isolated bioactive components.
Solution Approach 2:
The patent creates a functional copy of cell activity through non-cellular preparations that replicate regenerative effects without containing actual cells. The extracellular matrix and growth factor combinations mimic cellular functions, providing regenerative capacity through molecular copies rather than biological originals.
2Object-affected harmful factors
If antigenic properties are removed to prevent inflammation, then safety is improved, but regenerative capacity is diminished
Solution Approach 1:
The patent removes antigenic cell components while retaining non-cellular bioactive elements that mediate regenerative functions. By extracting whole cells that cause immune reactions while preserving extracellular matrix and growth factors, the preparation achieves both safety and regenerative capacity.
Solution Approach 2:
The patent uses non-cellular components as intermediary substances that transmit regenerative signals without triggering immune responses. Growth factors and extracellular matrix proteins act as mediators that promote tissue repair while lacking the antigenic properties of whole cells.
3Reliability
If whole cells are used for tissue regeneration, then regenerative capacity is maintained, but inflammation and immune rejection occur
Solution Approach 1:
The patent extracts and removes the harmful cellular components that cause inflammation and immune rejection, while retaining the beneficial non-cellular elements that provide regenerative capacity. This separation eliminates harmful effects while preserving useful functions.
Solution Approach 2:
The patent creates a non-cellular copy that reproduces regenerative functions without the harmful immune properties of actual cells. The extracellular matrix and growth factor preparation serves as a functional analogue that maintains regenerative capacity while eliminating antigenicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition achieves safe and effective regenerative support, sustaining tissue formation and morphology, independent of mesenchymal cells, with enhanced vitality and reduced inflammation, maintaining regenerative capacity through sub-freezing or non-cryogenic preservation methods.
Implementation Method 1
The biological composition is treated in a protectant or cryoprotectant prior to preservation or cryopreservation or freeze drying
Implementation Method 2
The protectant or cryoprotectant creates a physical or electrical or chemical gradient or combination thereof for tissue regeneration
Implementation Method 3
the cryopreservation occurs at a temperature that is sub-freezing wherein the cryopreservation temperature is from 0 degrees C. to −200 degrees C.
Data Source
AI summary
A biological composition has a mixture of mechanically selected allogeneic biologic material derived from placental tissue. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.