Placental Stem Cell Collection Composition with Proteases and Perfluorocarbons
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Solution Overview
Problem
Current methods for collecting stem cells from placentas are inefficient, leading to apoptosis or necrosis due to environmental changes during collection and storage, resulting in a low yield of viable stem cells.
Innovation Solution
The use of a physiologically-acceptable aqueous solution containing proteases, JNK inhibitors, immunomodulatory compounds, and oxygen-carrying perfluorocarbons to facilitate the perfusion and enzymatic disruption of placentas, reducing apoptosis and necrosis and improving stem cell recovery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current collection methods are used, then the collection process is simple, but the yield of viable stem cells is low due to apoptosis and necrosis
Solution Approach 1:
The composition is prepared in advance with proteases, JNK inhibitors, and other protective agents before stem cell collection. This preliminary preparation ensures that when collection occurs, the stem cells are immediately protected from environmental stressors that would otherwise cause apoptosis and necrosis, thereby increasing viable yield without complicating the actual collection procedure
Solution Approach 2:
The composition acts as an intermediary medium between the stem cells and the harsh collection environment. It mediates protection by containing proteases that prevent protein degradation, JNK inhibitors that block apoptosis signaling, and other agents that collectively shield stem cells from stressors during collection and storage
2Reliability
If environmental changes occur during collection and storage, then the collection process is straightforward, but apoptosis and necrosis increase
Solution Approach 1:
The composition provides beforehand cushioning by incorporating protective agents that anticipate and prevent environmental damage. Proteases prevent protein degradation, JNK inhibitors block apoptosis pathways, and other protective compounds are included in advance to cushion stem cells against the harsh conditions of collection and storage, ensuring high viability without complicating the procedure
3Reliability
If proteases and inhibitors are added to the composition, then stem cell preservation is improved, but the composition complexity increases
Solution Approach 1:
Multiple protective functions are merged into a single composition. Proteases, JNK inhibitors, and other protective agents are combined in one formulation that can be applied during collection. This merging provides comprehensive protection against multiple degradation pathways simultaneously without requiring separate treatment steps, thus improving preservation while maintaining procedural simplicity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the collection and preservation of stem cells by maintaining their viability and preventing apoptosis, resulting in a higher yield of functional stem cells.
Implementation Method 1
The compositions disclosed herein comprise a physiologically-acceptable aqueous solution, e.g., a saline solution, one or more proteases and one or more inhibitors of JNK
Implementation Method 2
Optionally, the composition further comprises a compound that modulates (e.g., inhibits) TNF-α, an immunomodulatory compound, a vasodilator, necrosis inhibitor, oxygen-carrying perfluorocarbon
Data Source
AI summary
The present invention provides improved compositions and methods for the collection of stem cells from an organ, e.g., placenta. The invention provides a stem cell collection composition comprising an apoptosis inhibitor and, optionally, an enzyme such as a protease or mucolytic enzyme, vasodilator, necrosis inhibitor, oxygen-carrying perfluorocarbon, or an organ preserving compound. The invention provides methods of using the stem cell collection composition to collect stem cells and to preserve populations of stem cells.