Natural coloring agent composition

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Solution Overview

Problem

Existing methods for producing natural nitrites in South Korea are inadequate in securing sufficient amounts and efficient reduction technologies, leading to challenges in developing natural coloring agents for meat products that provide effective color development without synthetic additives.

Innovation Solution

A natural coloring agent composition is developed using a fermented plant product containing high concentrations of nitrite, produced by activating nitrate reductase within the plant, which includes free sugar in limited amounts to prevent browning and includes various amino acids and organic acids for improved taste and color development.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If external reducing bacteria are used to produce natural nitrites, then nitrite production is achieved, but production cost increases and unintended substances may be generated

Engineering Contradiction:
Improvenitrite concentrationVSAvoidunintended substances
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The invention extracts and utilizes the nitrate reductase enzyme from plant sources (such as spinach) to convert nitrate to nitrite, separating the desired enzymatic function from the bacterial cells themselves. This allows nitrite production without introducing live bacteria that could generate unintended substances, while maintaining natural nitrite generation through plant-based enzymatic activity

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention introduces plant-derived nitrate reductase enzyme as an intermediary substance that mediates the conversion of nitrate to nitrite. This enzyme acts as a catalyst to achieve nitrite production without requiring the presence of external reducing bacteria, thereby preventing the generation of unintended bacterial byproducts while maintaining efficient nitrite generation

Inventive Principle:
Principle #24Intermediary (Mediator)

2Quantity of substance

If external reducing bacteria are inoculated for nitrite production, then nitrite is produced, but production process complexity and cost increase

Engineering Contradiction:
Improvenitrite concentrationVSAvoidproduction process simplicity
Core Design Contradiction:
Quantity of substanceVSEase of manufacture

Solution Approach 1:

The invention extracts only the essential nitrate reductase enzyme from plant sources, eliminating the need for complex bacterial cultivation, inoculation, and control processes. This simplifies the manufacturing process by using a stable plant-based enzyme preparation instead of live bacteria, reducing production complexity while maintaining nitrite production efficiency

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention uses plant-based enzyme preparations that can be produced through simple fermentation or extraction processes, replacing the need for maintaining complex bacterial cultures. This approach uses readily available plant materials and simplifies the production workflow, making the process more economically viable and easier to manufacture

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

3Illumination intensity

If conventional natural coloring agents are used, then color development is achieved, but residual nitrite ions are high causing health concerns

Engineering Contradiction:
Improvecolor development effectVSAvoidresidual nitrite ions
Core Design Contradiction:
Illumination intensityVSObject-affected harmful factors

Solution Approach 1:

The invention optimizes the concentration of nitrate reductase enzyme and reaction conditions (pH, temperature, reaction time) to achieve complete conversion of nitrate to nitrite, thereby minimizing residual nitrite ions. By carefully controlling these parameters, the process achieves effective color development while reducing harmful residual nitrite levels to safe ranges

Inventive Principle:
Principle #35Parameter changes

4Illumination intensity

If high concentration of nitrite is achieved for effective color development, then color effect is improved, but health risks from residual nitrite increase

Engineering Contradiction:
Improvecolor development effectVSAvoidhealth risks from residual nitrite
Core Design Contradiction:
Illumination intensityVSObject-affected harmful factors

Solution Approach 1:

The invention optimizes reaction parameters including enzyme concentration, pH, temperature, and reaction time to achieve maximum nitrite generation efficiency. By carefully controlling these parameters, the process ensures sufficient nitrite concentration for effective color development while minimizing excess residual nitrite that could pose health risks

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition achieves color development in meat products similar to synthetic nitrites, reduces browning, and enhances taste quality while minimizing health risks and production costs by eliminating the need for bacterial inoculation and reducing bacteria by-products.

Implementation Method 1

a natural coloring agent composition which includes a fermented plant product, includes nitrite in a concentration of 700 ppm or more

Methodology Applied
Scientific EffectNitrate reductase enzyme reaction: Enzyme

Implementation Method 2

a natural coloring agent composition including a fermented plant product

Methodology Applied
Scientific EffectFermentation: Fermentation

Data Source

PatentUS12624213B2Natural coloring agent composition
Publication Date: 2026.05.12 CJ CHEILJEDANG CORP
  • US12624213B2 patent drawing

AI summary

The present application relates to a natural coloring agent composition including a fermented plant product, wherein the composition includes nitrite in a concentration of 700 ppm or more and includes free sugar in a concentration of 1,000 ppm or less, and the free sugar includes lactose. The natural coloring agent composition may be prepared from natural raw materials to contain nitrite in a high concentration without inoculation of reducing bacteria, and may have an excellent color development effect when applied to meat products in place of synthetic nitrite.