Plant Protein Liquid Processing for Solubility and Digestibility

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Solution Overview

Problem

Plant protein-containing liquid compositions face challenges in solubility, digestibility, and mineral solubility, necessitating improvements to enhance nutrient absorption and food characteristics.

Innovation Solution

A method involving the use of a protein deamidase and cellulase treatment to improve solubility, digestibility, and mineral solubility of plant proteins, with specific enzyme combinations and conditions optimized for efficacy.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If plant protein-containing liquid composition is treated with protein deamidase alone, then protein solubility is improved, but digestibility and mineral solubility remain insufficient

Engineering Contradiction:
Improveprotein solubilityVSAvoiddigestibility and mineral solubility
Core Design Contradiction:
Quantity of substanceVSReliability

Solution Approach 1:

The patent combines protein deamidase treatment with cellulase treatment in a sequential processing method. The protein deamidase modifies protein structure to improve solubility, while the subsequent cellulase treatment breaks down cellulose matrices to further enhance digestibility and mineral solubility. This merging of two enzymatic treatments resolves the contradiction by achieving all three targets simultaneously.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The protein deamidase treatment is performed as a preliminary step before cellulase treatment. This preliminary action of deamidation modifies the protein structure in advance, making it more soluble and accessible, which then allows the subsequent cellulase treatment to more effectively improve digestibility and mineral solubility.

Inventive Principle:
Principle #10Preliminary action

2Quantity of substance

If protein solubility is improved by treating with protein deamidase and protease, then solubility increases, but protein hydrolysis occurs which may affect food characteristics

Engineering Contradiction:
Improveprotein solubilityVSAvoidprotein hydrolysis affecting mouthfeel
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The patent extracts or removes the protease treatment step from the processing sequence, retaining only protein deamidase and cellulase treatments. This extraction eliminates excessive protein hydrolysis that would degrade mouthfeel, while still achieving improved solubility through deamidation and enhanced digestibility through cellulose breakdown.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent changes the enzymatic treatment parameters by selecting specific enzymes (protein deamidase and cellulase) with specific activities and controlling their amounts and treatment conditions. This parameter optimization allows solubility improvement without excessive hydrolysis that would harm food characteristics.

Inventive Principle:
Principle #35Parameter changes

3Adaptability or versatility

If plant milk is used as alternative to animal milk, then dietary restrictions are addressed, but protein content and nutrient absorption efficiency remain lower

Engineering Contradiction:
Improvedietary alternative capabilityVSAvoidprotein content and nutrient absorption
Core Design Contradiction:
Adaptability or versatilityVSQuantity of substance

Solution Approach 1:

The patent combines protein deamidase treatment with cellulase treatment to simultaneously improve protein solubility, digestibility, and mineral solubility in plant milk. This merged enzymatic approach increases the bioavailability and absorption efficiency of proteins and minerals, making plant milk a more nutritious alternative to animal milk while maintaining its dietary adaptability.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The treatment significantly enhances the solubility, digestibility, and mineral solubility of plant proteins, allowing for more efficient nutrient absorption and improved food characteristics.

Implementation Method 1

treating a plant protein-containing liquid composition with a protein deamidase and cellulase

Methodology Applied
Scientific EffectDeamidation: Decomposition (biological)

Implementation Method 2

treating a plant protein-containing liquid composition with a protein deamidase and cellulase

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Data Source

PatentEP4666873A1Production method for processed vegetable protein-containing liquid composition
Publication Date: 2025.12.24 AMANO ENZYME INC
  • EP4666873A1 patent drawing
  • EP4666873A1 patent drawing
  • EP4666873A1 patent drawing

AI summary

The purpose of the present invention is to provide a processing technology that improves the solubility and/or digestibility of a protein in a plant protein-containing liquid composition. The solubility, digestibility, and/or mineral solubility of a protein in a plant protein-containing liquid composition can be improved by treating the plant protein-containing liquid composition with a protein deamidase and a cellulase.