Plant Regulatory Elements for Gene Expression Control
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Solution Overview
Problem
Current plant genetic engineering lacks effective gene regulatory elements that can efficiently modulate gene expression in plants, particularly for agronomically important traits like herbicide resistance and pest resistance, and there is a need for diverse regulatory elements to achieve specific expression patterns.
Innovation Solution
Development of novel DNA regulatory elements, including promoters, leaders, and introns, with high sequence identity and gene-regulatory activity, which are operably linked to heterologous transcribable polynucleotide molecules to create transgenic plants capable of expressing genes of agronomic interest.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional plant genetic engineering methods are used, then basic gene expression can be achieved, but efficient modulation of gene expression for agronomically important traits is lacking
Solution Approach 1:
The patent applies parameter changes by providing regulatory elements with specific sequence characteristics (e.g., GC content, length, motif composition) that can be adjusted to control gene expression levels and patterns. The regulatory elements are designed with specific parameters such as promoter strength, enhancer positioning, and insulator sequences to achieve reliable expression modulation for agronomic traits.
Solution Approach 2:
The patent uses composite regulatory elements that combine multiple functional components (promoters, enhancers, silencers, insulators) into integrated DNA constructs. These composite regulatory elements work together to achieve precise control over gene expression, enabling both high productivity and reliable modulation for traits like herbicide resistance and pest resistance.
2Adaptability or versatility
If diverse regulatory elements are introduced to achieve specific expression patterns, then expression control versatility improves, but construct complexity increases
Solution Approach 1:
The patent applies segmentation by dividing the regulatory element into distinct functional modules (promoter, enhancer, silencer, insulator) that can be independently designed and combined. This modular approach allows for versatile expression pattern control while managing complexity through standardized components that can be assembled in different configurations for different agricultural applications.
3Productivity
If regulatory elements are operably linked to heterologous transcribable polynucleotide molecules, then gene modulation capability is enhanced, but sequence identity requirements increase
Solution Approach 1:
The patent addresses sequence identity requirements by providing regulatory elements with specific sequence characteristics that can be optimized for heterologous expression. The regulatory elements are designed with conserved functional motifs and adaptable sequence parameters that maintain effectiveness across different plant species, enabling enhanced gene modulation capability while managing sequence identity constraints.
Data Source
AI summary
The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.