Plant Urease Soil Solidification via Protein Removal
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Solution Overview
Problem
The existing plant urease-based soil solidification agents face issues with high protein content causing soil clogging and reduced permeability, and there is a need for an economical and zero-waste production process to improve their effectiveness and reduce costs.
Innovation Solution
A pretreated plant urease-containing soil solidification agent is developed using a zero-waste production process that involves optimizing the pretreatment of plant urease solutions to remove proteins, using a specific ratio of plant urease to inorganic treatment solutions, and recycling by-products to create a more effective and cost-efficient soil solidification agent.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If plant urease is extracted from plant tissues using simple crude purification, then the extraction process is easy and rapid, but the resulting solution contains high protein content that causes soil clogging and reduces permeability
Solution Approach 1:
The patent extracts and removes proteins from the plant urease solution through precipitation and filtration processes. The protein precipitation agent causes proteins to precipitate out of the solution, which are then removed through filtration, thereby eliminating the harmful effect of protein-induced soil clogging while retaining the useful urease enzyme.
Solution Approach 2:
The patent introduces a protein precipitation agent as an intermediary substance that facilitates the separation of proteins from the urease solution. This intermediary causes the proteins to precipitate and can be easily removed through filtration, solving the problem of protein contamination without affecting the urease activity.
2Productivity
If plant urease solution is used directly without protein removal, then the production process is simple and cost-effective, but the proteins block voids among soil particles and hinder infiltration
Solution Approach 1:
The patent performs preliminary protein removal treatment on the plant urease solution before it is applied to the soil. By conducting the protein precipitation and filtration steps in advance, the solution is pre-treated to eliminate proteins that would otherwise block soil voids and hinder infiltration during the actual soil solidification process.
3Power
If commercial urease is used, then the enzyme activity is high, but the purification process is difficult and high-cost
Solution Approach 1:
The patent uses inexpensive plant-based urease sources (such as soybeans, peas, or other legumes) instead of expensive commercial urease preparations. By utilizing cheap plant materials and simple extraction methods, the patent achieves cost-effective production while maintaining sufficient enzyme activity for soil solidification applications.
4Object-affected harmful factors
If proteins are removed from plant urease solution, then soil permeability is improved, but the production process becomes more complex
Solution Approach 1:
The patent changes the physical-chemical parameters of the plant urease solution by adjusting pH, temperature, and the addition of precipitation agents to optimize protein precipitation. By controlling these parameters, the patent achieves effective protein removal through simple filtration processes, balancing improved soil permeability with manageable production complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process significantly reduces protein concentration, improves infiltration, broadens the application range of the soil solidification agent, and achieves zero-waste production, reducing environmental impact and costs by reusing soybean proteins and bean dregs to produce valuable by-products.
Implementation Method 1
adding a protein precipitation agent to the plant urease solution to precipitate proteins, and filtering the precipitated proteins from the plant urease solution
Implementation Method 2
filtering the precipitated proteins from the plant urease solution
Implementation Method 3
urea, a calcium source, and a commercial urease are added to soil at a predetermined ratio to induce the formation of calcium carbonate with cementing capacity
Implementation Method 4
plant urease can be a relatively perfect substitute for commercial urease in an EICP experiment... decompose urea into carbonate
Implementation Method 5
induce the formation of calcium carbonate with cementing capacity, such that soil particles are cemented together
Data Source
AI summary
The present invention belongs to the technical field of rock and soil reinforcement, and specifically relates to a plant urease-containing soil solidification agent and a zero-waste production process thereof. A crudely-purified plant urease solution includes a large amount of irrelevant proteins, which seriously affects the treatment effect of the soil solidification agent, makes it impossible to achieve an expected construction purpose, and also causes serious waste and environmental pollution. In the present invention, a common plant urease extraction method on the market is optimized to significantly reduce a protein concentration in a plant urease solution, greatly improve the infiltration performance of a plant urease-containing soil solidification agent, and broaden an application range of such a soil solidification agent.