Direct Plasma Purification Using 4-15 μm Chromatography
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Solution Overview
Problem
Current methods for purifying target components from full plasma samples require pre-processing steps such as dilution and cryoprecipitation, which increase process time and cost, and risk clogging chromatographic units or activating plasma components.
Innovation Solution
A method involving the direct application of full, undiluted plasma to a continuous chromatographic material with channels of 4 to 15 μm diameter, using a chromatographic selection element, and performing chromatography at 30 to 39°C to recover target components without pre-processing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If sample dilution is performed before plasma processing, then plugging of chromatographic units is prevented, but process time increases and cost increases
Solution Approach 1:
The patent changes the physical parameters of the plasma sample by adjusting pH and temperature, and by performing ultrafiltration to concentrate the sample. These parameter changes enable direct injection of plasma without dilution, preventing chromatographic unit plugging while avoiding the time loss associated with dilution steps
2Reliability
If sample dilution is performed before plasma processing, then chromatographic unit plugging is prevented, but process cost increases
Solution Approach 1:
The patent employs parameter changes including pH adjustment, temperature control, and ultrafiltration concentration to prepare plasma for direct injection. This eliminates the need for dilution with expensive buffers and reduces overall process cost while maintaining reliability by preventing plugging
3Reliability
If plasma is processed at low temperatures, then degradation of sensitive molecules is prevented, but viscosity increases causing processing difficulties
Solution Approach 1:
The patent performs preliminary ultrafiltration and concentration of plasma before chromatographic analysis. This preliminary action removes excess water and concentrates the sample, which compensates for the increased viscosity at low temperatures and enables successful processing without degradation of sensitive molecules
4Loss of time
If direct injection of undiluted plasma is performed, then process time is reduced and cost is reduced, but chromatographic unit plugging occurs
Solution Approach 1:
The patent applies parameter changes including pH adjustment, temperature control, and ultrafiltration concentration to prepare undiluted plasma for direct injection. These changes modify the plasma properties to prevent plugging while maintaining the time and cost advantages of direct injection without dilution
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables efficient purification of target components from full plasma without clogging or activating plasma components, reducing process time and cost, and improving the yield and quality of the product.
Implementation Method 1
a continuous chromatographic material such as a synthetic monolith or membrane-based support for the processing of full, undiluted human plasma
Implementation Method 2
the chromatographic material comprising a chromatographic selection element for the target component
Implementation Method 3
the continuous chromatographic material comprising channels having a diameter of 4 to 15 μm
Data Source
AI summary
The present invention relates to a method of purifying a target component from a full plasma sample. The present invention further relates to a method of removing one or more target components from a full plasma sample.