Aqueous Two-Phase Plasma Separation for Regenerative Factor Concentration
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Solution Overview
Problem
Existing methods fail to effectively concentrate and extract regenerative factor-rich plasma (RRP) from blood using an aqueous two-phase system, and there is a lack of therapeutic applications for regenerative factors due to their heterogeneous nature and challenges in isolation and purification.
Innovation Solution
A method involving centrifugation and chromatography steps to extract and concentrate RRP using specific concentrations of sodium sulfate, PEG, and hyaluronic acid, followed by purification with chromatography filters and selective extraction using anti-regenerative factor antibodies, along with a device for implementing this process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional methods are used to extract regenerative factors from blood, then the extraction process is simple, but the concentration of regenerative factors in the extracted plasma is insufficient
Solution Approach 1:
The extraction process is divided into multiple sequential steps: initial plasma separation, aqueous two-phase system formation, and selective regenerative factor concentration. Each step isolates and concentrates specific components, achieving high overall concentration (up to 10 times) while managing complexity through modular processing stages
Solution Approach 2:
An aqueous two-phase system comprising polymers and kosmotropic salts is introduced as an intermediary medium to facilitate selective partitioning of regenerative factors from blood plasma. This intermediary system enables high-concentration extraction without requiring direct complex interaction between all blood components and the final product
2Productivity
If an aqueous two-phase system is used to concentrate regenerative factors, then the concentration efficiency increases significantly, but the types and concentrations of substances required become critical and difficult to optimize
Solution Approach 1:
The invention systematically optimizes parameters including polymer concentration (e.g., 10-20% w/v), kosmotropic salt concentration (e.g., 0.5-2.0 M), pH (e.g., 6.0-8.0), and temperature to achieve reliable aqueous two-phase system formation. These parameter specifications make the process reproducible and manufacturable while maintaining high concentration efficiency
Solution Approach 2:
Blood is pre-treated through plasma separation and filtration before introducing the aqueous two-phase system. This preliminary preparation removes interfering components and standardizes the input material, making subsequent phase separation more predictable and easier to manufacture at scale
3Manufacturing precision
If multiple purification steps are performed to achieve high purity RRP, then the purity of regenerative factors increases, but the processing time and complexity increase
Solution Approach 1:
Multiple purification functions are merged into the aqueous two-phase system process, which simultaneously performs separation, concentration, and purification in a single integrated operation. This reduces the need for sequential purification steps while achieving high purity (up to 95% or higher) regenerative factor-rich plasma
Solution Approach 2:
The aqueous two-phase system exploits liquid-liquid phase separation to purify regenerative factors. Target proteins partition preferentially into one phase based on their physicochemical properties, achieving purification through natural phase transition without requiring multiple mechanical filtration or chromatography steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves a high yield of RRP, concentrating regenerative factors like BMP, FGF, and PDGF up to 10 times more than whole blood, enabling therapeutic applications in wound treatment and cosmetic uses.
Implementation Method 1
Aqueous two-phase system is a method of concentrating and extracting particles by creating two aqueous phases
Implementation Method 2
a main body (30) connected to the lid and performing centrifugation to separate RRP
Data Source
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AI summary
More specifically, the method enables the efficient extraction and concentration of regeneration factor-rich plasma (RRP) with regenerative efficacy in a short time, and the RRP manufactured using this method promotes cell proliferation, making it applicable for regenerative therapy and skin beauty.