Plasma-Treated Cell Culture Carrier for Bioreactors
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Solution Overview
Problem
Current cell culture carriers are inadequate for isolating, multiplying, and harvesting NK cells, T lymphocytes, and mesenchymal stem cells in sufficient quantities for effective cell therapy, as they often result in low cytotoxicity and unsuitable cell yields for therapeutic applications.
Innovation Solution
A cell culture carrier with a hydrophilic carrier body treated with low-temperature plasma and a cell-adhesive coating containing fibronectin, retronectin, collagen, gelatin, laminin, and activating antibodies such as immunoglobulins and anti-CD antibodies, suitable for use in various bioreactor types, is developed to enhance cell adhesion and proliferation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional cell culture carriers are used, then cell cultivation is possible, but cell yield and cytotoxicity are insufficient for effective cell therapy
Solution Approach 1:
The patent applies parameter changes by modifying the surface properties of the cell culture carrier through specific coatings (fibronectin, retronectin, collagen, gelatin, laminin) and plasma treatment. These changes in surface chemistry and physics parameters enable enhanced cell adhesion and activation, directly addressing the insufficient cell yield and therapeutic effectiveness of conventional carriers
Solution Approach 2:
The patent employs composite materials by combining multiple coating layers with different functions on the carrier surface. The carrier body is treated with low-temperature plasma and coated with a combination of adhesive proteins (fibronectin, retronectin, collagen, gelatin, laminin) and activating antibodies, creating a multi-functional composite surface that simultaneously improves cell attachment and activation for higher cell yield and therapeutic effectiveness
2Productivity
If cell adhesion is enhanced for high-density cultivation, then cell proliferation improves, but cell stress and harvesting difficulty increase
Solution Approach 1:
The patent uses parameter changes by controlling the physical and chemical properties of the coating materials and their deposition conditions. The low-temperature plasma treatment and controlled coating application create optimal adhesion parameters that support high cell density without causing excessive mechanical stress or making cell detachment difficult
Solution Approach 2:
The patent applies porous materials by using a porous carrier body structure that provides increased surface area for cell attachment while maintaining mechanical flexibility. The porous structure allows cells to attach on the surface and within the pores, enabling high-density cultivation without excessive cell stress and facilitating easier harvesting
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables efficient isolation, activation, and harvesting of immune cells, particularly NK cells and tumor-infiltrating lymphocytes, which can be used therapeutically with high specificity against tumor cells and metastases, achieving significant cell growth and separation with minimal stress.
Implementation Method 1
a hydrophilic carrier body (21) treated with low-temperature plasma
Data Source
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AI summary
The invention relates to a cell culture carrier for bioreactors for the isolation, multiplication and harvesting of cells, in particular NK cells, T lymphocytes and tumor-infiltrating lymphocytes (TIL) and mesenchymal stem cells from bone marrow, wherein the carrier has a coating specific to the respective cell type (adhesive; receptor/ligand; co-receptor, etc.).