Plasmodiophora-Responsive Promoter for Targeted Plant Immunity Expression
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Solution Overview
Problem
Cruciferous plants like Brassica napus are severely affected by Plasmodiophora diseases, which impact yield and quality, and existing plant resistance genes, such as NLR and Executor R genes, have limitations in activation mechanisms and spatiotemporal expression specificity.
Innovation Solution
A Plasmodiophora-responsive promoter, represented by the RCR1 Est< promoter or variants with 50% or more identity, is used to drive gene expression and enhance resistance, integrated into expression cassettes and vectors for transgenic plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing plant resistance genes (NLR or Executor R genes) are used, then plant immunity is activated, but the activation mechanisms differ and lack spatiotemporal expression specificity
Solution Approach 1:
The promoter contains specific cis-acting elements (such as W-box, TGA-element, and others) that are locally distributed at specific positions within the promoter sequence. These local elements bind to specific transcription factors (like TGA2.1) to regulate gene expression in a spatiotemporal manner, providing expression specificity that differs from the uniform activation mechanism of traditional NLR or Executor R genes.
Solution Approach 2:
The invention changes the regulatory parameters of gene expression by using a promoter with specific cis-acting elements that respond to Plasmodiophora infection. This allows dynamic adjustment of expression levels and timing based on pathogen presence, rather than constitutive or uniformly induced expression of traditional resistance genes.
2Reliability
If Executor R genes are used to directly kill infected cells, then resistance is achieved, but transcriptional regulation lacks responsiveness to specific pathogen effectors
Solution Approach 1:
The promoter contains cis-acting elements that form a feedback regulatory system with transcription factors. When Plasmodiophora effectors are detected, they trigger signaling cascades that activate specific transcription factors, which then bind to the promoter elements to induce resistance gene expression. This creates a responsive feedback loop that adjusts gene expression based on pathogen presence, unlike the constitutive or non-specific induction of traditional Executor R genes.
3Reliability
If NLR genes are used to activate immunity through effector recognition, then specific resistance is achieved, but the auto-inhibited state limits rapid response
Solution Approach 1:
The promoter is pre-configured with specific cis-acting elements and transcription factor binding sites that are ready for immediate activation upon pathogen detection. The transcriptional machinery and regulatory elements are in place beforehand, allowing rapid gene induction when Plasmodiophora effectors are detected, without the delay associated with NLR protein conformational changes and activation.
Data Source
Figure 1A~2C
Figure 3A~3C
AI summary
The present invention discloses a plant Plasmodiophora-responsive promoter and the use thereof. The plant Plasmodiophora-responsive promoter is A1) a DNA molecule as shown in sequence 3, or A2) a DNA molecule having 50% or more identity to the nucleotide sequence as defined in A1) and having promoter function. It is proved by experiments that the promoter disclosed in the present invention comprises important elements responsive to Plasmodiophora infection, and is a Plasmodiophora-responsive promoter. The promoter is very important for mediating resistance to Plasmodiophora, and has an important application value.