Plastic Fluid Microbial Composition for Sedimentation-Free Storage
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Solution Overview
Problem
Existing methods for storing microorganisms, such as subculture, cryopreservation, and freeze-drying, face issues with contamination, sedimentation, low survival rates, and inefficiencies in use, particularly in liquid-type microbial preparations, which are costly and require frequent stirring.
Innovation Solution
A plastic fluid composition containing microorganisms and polysaccharides or nanofibers, with specific yield values and viscosities, maintains microorganisms in a suspended state without sedimentation for extended periods, allowing high-density storage and use without special treatment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If liquid-type microbial preparations are used for storage, then storage period and use form are improved, but microorganisms settle and form aggregates causing quality deterioration
Solution Approach 1:
The patent changes the physical-chemical parameters of the storage medium by incorporating specific additives and adjusting composition to modify viscosity and suspension properties, preventing microbial settling while maintaining liquid state storage
Solution Approach 2:
The invention uses composite storage media combining multiple components including buffers, carbohydrates, and specific additives that work together to maintain microbial suspension stability without causing aggregation or settling
2Stability of the object's composition
If microorganism concentration is lowered to suppress settling and aggregation, then sedimentation is reduced, but storage space and transportation costs increase
Solution Approach 1:
The patent modifies the physical parameters of the storage medium to increase viscosity and improve suspension characteristics, allowing high concentrations of microorganisms to remain stably suspended without settling, thus maintaining both high storage efficiency and suspension stability
3Stability of the object's composition
If stirring is performed at regular intervals to maintain quality, then microbial distribution is improved, but physical stress and contamination risk increase
Solution Approach 1:
The invention creates a self-sustaining storage system where the modified composition inherently maintains microbial suspension through its physical-chemical properties, eliminating the need for external stirring interventions and thereby reducing contamination risk while preserving uniform distribution
4Duration of action of stationary object
If freeze-drying method is used for storage, then preservation properties are improved, but microorganisms are exposed to freezing and drying stress reducing survival rate
Solution Approach 1:
The patent changes the storage conditions from extreme freeze-drying parameters to milder liquid-state parameters with optimized temperature, viscosity, and chemical composition, achieving long-term preservation while avoiding the damaging effects of freezing and drying stress on microorganisms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition supports high survival rates and functional stability of microorganisms under various conditions, suppressing contamination and sedimentation, enabling efficient storage and use of multiple types without stirring.
Implementation Method 1
a plastic fluid having a yield value of 5 to 500 mPa and a viscosity of 1.5 to 200 mPa·s at 4 to 25° C.
Implementation Method 2
a plastic fluid having a yield value of 5 to 500 mPa and a viscosity of 1.5 to 200 mPa·s at 4 to 25° C.
Data Source
AI summary
The invention provides a plastic fluid composition containing (1) a microorganism and (2) a polysaccharide or a nanofiber composed of a polysaccharide, wherein when the microorganism is a bacterium, it is comprised at a microbial density of 1×107 CFU/mL or more, and is dispersed in the composition, and when the microorganism is a fungi, it is comprised at a microbial density of 1×103 CFU/mL or more, and is dispersed in the composition.