Plate Contamination Detection from Nucleic Acid Well Patterns
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for detecting contamination in molecular diagnostic tests, such as cross-contamination between reaction wells, are time-consuming and costly, leading to incorrect results and delays in response, and there is a need for a more efficient method to determine contamination in nucleic acid amplification tests.
Innovation Solution
A method using a computer device to analyze detection results of reaction wells, determining the position of positive wells, and calculating contamination based on distance, density, and shape of these wells to identify contamination patterns, with the option to update contamination assumptions using re-detection results.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If retest is performed to check contamination, then measurement precision is improved, but loss of time and loss of energy increase
Solution Approach 1:
The system performs preliminary analysis of the detection result data by analyzing the positional relationships, density, and spatial distribution of positive reaction wells before concluding contamination. This preliminary computational assessment allows the system to identify contamination patterns without requiring additional physical retesting, thereby reducing time loss while maintaining detection accuracy.
Solution Approach 2:
The patent replaces the mechanical/repeat testing process with a computational analysis system that processes detection result data through algorithms. The system calculates contamination likelihood by evaluating spatial patterns of positive wells, substituting the time-consuming physical retest with a rapid computational assessment that achieves similar contamination detection goals.
2Productivity
If retest is skipped to reduce time loss, then productivity is improved, but measurement precision deteriorates due to incorrect test results
Solution Approach 1:
The system substitutes physical retesting with computational pattern recognition algorithms that analyze the spatial distribution, density, and positional relationships of positive reaction wells. This computational approach provides rapid contamination assessment without requiring additional testing cycles, maintaining both high productivity and accurate contamination detection through sophisticated data processing.
Solution Approach 2:
The system introduces an intermediary computational analysis layer between the initial test and the final contamination determination. This intermediary process evaluates detection result data through multiple criteria (positional relationships, density calculations, spatial patterns) to mediate the decision-making process, enabling rapid yet accurate contamination identification without direct retesting.
3Productivity
If contamination is not detected to avoid time loss, then productivity is improved, but object-affected harmful factors increase due to incorrect test results
Solution Approach 1:
The patent replaces time-consuming physical retesting with rapid computational analysis that processes detection result data through algorithms evaluating spatial patterns of positive wells. This substitution enables the system to detect contamination quickly and accurately, preventing incorrect test results from reaching examinees while maintaining high processing efficiency and productivity.
Solution Approach 2:
The system implements a feedback mechanism where the computational analysis continuously monitors the detection result data for contamination patterns. By analyzing positional relationships, density, and spatial distribution in real-time, the system provides immediate feedback on contamination status, enabling rapid intervention to prevent incorrect results while maintaining efficient test processing.
Data Source
AI summary
According to an embodiment, disclosed is a method for assuming a contamination of a plate used for a nucleic acid amplification test, performed by a computer device. The method may include: obtaining a detection result of a target analyte of a plurality of reaction wells in the plate; obtaining a position data of positive reaction wells in the plate from the detection result; and assuming the contamination of the plate by at least partially using the obtained position data.