Platelet-Associated Analyte Detection via Detergent Extraction
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Solution Overview
Problem
Current methods for determining platelet-associated analytes in samples are complex and not suitable for routine clinical laboratory processing, particularly for automated systems, as they require centrifugation and washing steps that are difficult to automate.
Innovation Solution
A method involving two test batches, one with platelet-rich plasma and a detergent, and another without, allows for the measurement of analyte activity, with the difference between the results indicating the platelet-associated analyte level, eliminating the need for additional centrifugation and washing steps.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional methods are used to determine platelet-associated analytes, then measurement precision is maintained, but device complexity and ease of operation deteriorate due to required centrifugation and washing steps
Solution Approach 1:
The invention extracts and measures only the platelet-associated fraction of the analyte by using a specific binding agent that selectively binds to platelets, allowing direct measurement from undiluted plasma without centrifugation or washing steps to separate platelets from plasma
Solution Approach 2:
The binding agent serves multiple functions: it selectively binds to platelets, concentrates the platelet-associated analyte, and enables direct measurement in a single step, replacing multiple separate operations (centrifugation, washing, resuspension) with one universal reagent
2Measurement precision
If traditional methods are used to determine platelet-associated analytes, then measurement precision is maintained, but ease of operation deteriorates due to manual centrifugation and washing steps
Solution Approach 1:
The method extracts the platelet-associated analyte signal directly from plasma using a binding agent that specifically targets platelets, eliminating the need for manual centrifugation and washing steps that complicate the operation
Solution Approach 2:
The binding agent performs the separation and concentration functions automatically through its selective binding properties, making the process self-service and eliminating manual intervention for centrifugation, washing, and resuspension steps
3Measurement precision
If traditional methods are used to determine platelet-associated analytes, then measurement precision is maintained, but extent of automation deteriorates due to difficult-to-automate centrifugation and washing steps
Solution Approach 1:
The invention extracts the platelet-associated analyte using a binding agent that enables direct measurement from plasma, removing the centrifugation and washing steps that are difficult to automate
Solution Approach 2:
The mechanical separation process (centrifugation) is replaced by a chemical/biological binding process where the binding agent selectively binds to platelets, allowing automation without complex mechanical separation steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method simplifies the detection of platelet-associated analytes, making it suitable for routine clinical laboratory processing and automated systems, providing precise classification of conditions like von Willebrand syndrome by accurately measuring platelet-associated substances without complex sample preparation.
Implementation Method 1
providing a first test batch containing platelet-rich plasma of an individual and a detergent
Data Source
AI summary
The present invention relates to a routine in vitro method for the determination of platelet-associated analytes, such as VWF, factor XIII, fibrinogen or D-dimer in a sample of platelet-rich plasma.