Blood Analyzer Platelet Detection Using Segmented Impedance and Optical Verification
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Solution Overview
Problem
Automated blood analyzers using the electrical impedance principle struggle to differentiate between particles of similar volume, such as large platelets, red blood cell fragments, and microcytes, leading to false counting results and inaccuracies in platelet detection due to incomplete cleaning of detection channels and particle attachment.
Innovation Solution
A method and system that involves mixing a blood sample with a diluent agent and a lytic reagent containing a hemolytic agent to create separate test samples for electrical impedance and optical detection, using forward and side scattered light signals, and fluorescent signals to differentiate platelets from other cell types, allowing for accurate platelet detection and alarming abnormal detection results.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If electrical impedance detection is used for platelet counting, then the detection process is simple and fast, but the measurement precision deteriorates due to inability to differentiate particles of similar volume
Solution Approach 1:
The patent segments the detection process into two independent channels: electrical impedance detection for rapid platelet counting and optical detection for accurate particle differentiation. By dividing the detection function across different physical principles, the system maintains high throughput while improving measurement precision through optical verification of impedance-based measurements.
Solution Approach 2:
The patent introduces optical detection as an intermediary verification mechanism. The optical detection channel acts as a mediator that validates and corrects platelet counts by visually confirming particle identity, thereby improving measurement precision without significantly impacting the overall detection speed provided by the impedance channel.
2Device complexity
If electrical impedance detection is used without optical verification, then the device complexity is low, but the reliability deteriorates due to false counting of similar-volume particles
Solution Approach 1:
The detection system is segmented into two functional modules: a simple electrical impedance detection module for initial platelet counting and an optical detection module for reliability verification. This segmentation allows the system to maintain low complexity in the primary detection path while adding reliability through a separate verification path.
Solution Approach 2:
The optical detection channel provides feedback to verify and correct platelet count results from the electrical impedance channel. When optical verification confirms or corrects impedance-based measurements, the system achieves high reliability while maintaining relatively simple device architecture through intelligent data cross-validation.
3Productivity
If detection channel cleaning is insufficient between samples, then the productivity is high with rapid sequential detection, but the measurement precision deteriorates due to contamination from previous samples
Solution Approach 1:
Optical detection serves as an intermediary verification mechanism that identifies and flags contaminated measurements. By optically verifying particle characteristics, the system can detect contamination from previous samples and either correct or reject affected measurements, maintaining precision without sacrificing the high throughput enabled by rapid sequential impedance detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the accuracy of platelet detection by providing a reliable method to distinguish between different cell types and detect abnormalities in the analysis process, reducing false positives and improving the overall precision of blood analysis.
Implementation Method 1
the electrical impedance principle (also known as Coulter Principle). According to the electrical impedance principle, when particles suspended in an electrolyte pass through a detection aperture with the electrolyte, the equivalent resistance across the detection aperture will change
Implementation Method 2
detecting at least two types of optical signals of the second test sample; acquiring second platelet detection data of the blood sample based on the at least two types of optical signals
Implementation Method 3
detecting at least two types of optical signals of the second test sample
Data Source
AI summary
A method, system and storage medium for providing an alarm for indicating that an abnormality is present in a sample analyzer are provided. The method includes: mixing a first aliquot of a blood sample with a diluent agent to prepare a first test sample; mixing a second aliquot of the blood sample with a lytic reagent to prepare a second test sample; detecting electrical impedance signals of the first test sample; detecting at least two types of optical signals of the second test sample; acquiring first platelet detection data based on the electrical impedance signals; acquiring second platelet detection data based on the at least two types of optical signals; acquiring an evaluation result based on a difference between the first platelet detection data and the second platelet detection data; determining whether the evaluation result meets a preset condition to provide an alarm.