Platelet Lysate Bag Set for Volume-Reduced Production
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Solution Overview
Problem
Existing methods for producing platelet lysate are inefficient and require complex device structures, limiting their scalability and workability, especially when handling large volumes of platelet concentrates.
Innovation Solution
A method involving the collection of buffy coat from whole blood, centrifugation to extract a supernatant, removal of leukocytes, and subsequent centrifugation to concentrate platelets, followed by freezing and thawing to recover platelet lysate, using a system with a centrifuge and bag set to facilitate efficient production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If platelet concentrate is frozen and thawed to extract growth factor, then platelet lysate is obtained, but the device structure becomes complex and handling large volumes becomes inefficient
Solution Approach 1:
The production process is divided into separate functional modules: a concentration bag for platelet concentration, a freezing bag for frozen storage, and a thawing bag for controlled thawing. These segmented components allow each to be optimized independently, improving overall productivity without requiring a monolithic complex device structure.
Solution Approach 2:
A transfer mechanism serves as an intermediary between the concentration bag, freezing bag, and thawing bag. This intermediary component facilitates efficient material transfer between stages, enabling high-volume processing without direct complex integration between all components, thus maintaining modular simplicity while improving productivity.
2Quantity of substance
If large volumes of platelet concentrate are handled, then more platelet lysate can be produced, but refrigeration facility size requirements increase
Solution Approach 1:
The refrigeration requirement is segmented into two separate facilities: a freezing facility for concentrated platelets and a thawing facility for processed material. This segmentation allows each facility to be sized appropriately for its specific function rather than requiring one large facility to handle all volumes, enabling processing of large quantities without proportionally increasing single-facility size requirements.
Solution Approach 2:
Platelet concentration is performed beforehand in the concentration bag before freezing, reducing the volume that requires frozen storage. This preliminary concentration action means that the freezing facility needs to accommodate only the concentrated material rather than large volumes of dilute platelet concentrate, thereby reducing the required size of the freezing refrigeration facility while still enabling large-scale production.
3Volume of stationary object
If platelet concentrate volume is reduced before freezing, then smaller refrigeration facilities can be used, but additional processing steps are required
Solution Approach 1:
The concentration and freezing steps are merged into an integrated workflow where the concentration bag connects directly to the freezing bag. This merging eliminates the need for separate handling and transfer of large volumes, reducing refrigeration facility size requirements without requiring complex additional processing equipment - the existing centrifugation and freezing infrastructure is utilized in sequence.
Solution Approach 2:
The centrifugation device performs multiple functions: initial platelet separation, concentration of platelets in the concentration bag, and subsequent transfer to freezing bag. This multi-functionality reduces the need for separate specialized equipment for each step, maintaining device simplicity while enabling volume reduction before freezing to minimize refrigeration facility size.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for the efficient production of platelet lysate with a simple device structure, reducing the volume of concentrates to be frozen, enabling production from a large number of donors using small refrigeration facilities and ensuring good workability.
Implementation Method 1
centrifuging, following freezing and thawing, the platelet concentrate to recover a platelet lysate
Implementation Method 2
platelet separated from whole blood (WB) is frozen and thawed to break the cell membrane, thereby extracellularly extracting the growth factor in the granule
Data Source
AI summary
Provided are a method for producing a platelet lysate, a production system, and a bag set capable of efficiently producing a platelet lysate with a simple device structure. According to the method for producing a platelet lysate, by which a platelet lysate (PL) that contains a growth factor contained in platelet is produced, buffy coat is collected from whole blood; the buffy coat (BC) is centrifuged to extract a supernatant, thereby collecting a platelet concentrate from which leukocyte has been removed; and, prior to freezing, the platelet concentrate is centrifuged to remove the supernatant, thereby preparing a highly concentrated platelet concentrate. The highly concentrated platelet concentrate is then frozen and thawed, and further centrifuged to recover the platelet lysate.