Platelet Storage in CO2-Controlled Media to Reduce Storage Lesions
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Solution Overview
Problem
The short storage life of platelets under conventional storage conditions leads to high expiration-caused scrap rates, economic losses, and a shortage of platelets, primarily due to platelet storage lesions (PSLs) such as CD62P expression, PS eversion, glucose consumption, lactic acid accumulation, and reduced ATP production.
Innovation Solution
Regulating the partial pressure of carbon dioxide in the suspension medium for platelet storage to 30-50 mmHg, using a gas permeable or air-tight container with controlled gas fractions, and adding substances that produce carbon dioxide by chemical reaction to maintain optimal CO2 levels.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of moving object
If conventional storage under continuous shaking in air at 22±2°C is used, then platelets can be stored with simple equipment and operations, but the storage life is only 5 days with high expiration scrap rate
Solution Approach 1:
The patent changes the physical and chemical parameters of the storage environment, specifically adjusting the partial pressure of carbon dioxide to 30-50 mmHg and controlling temperature at 20-24°C, which transforms the storage conditions from conventional air shaking to a controlled gas environment, thereby extending storage life to 7 days or more
Solution Approach 2:
The patent introduces a suspension medium as an intermediary substance between platelets and the storage environment. This medium maintains optimal pH and partial pressure of carbon dioxide, acting as a buffer that protects platelets from storage lesions while extending storage duration
2Reliability
If conventional storage is used, then storage operations are simple, but platelet storage lesions occur including CD62P expression, PS eversion, glucose consumption, lactic acid accumulation, and reduced ATP production
Solution Approach 1:
The patent modifies critical storage parameters including raising partial pressure of carbon dioxide to 30-50 mmHg and controlling temperature at 20-24°C, which prevents platelet activation and metabolic deterioration, thereby maintaining platelet function quality and reducing storage lesions
Solution Approach 2:
The suspension medium serves as a protective intermediary that maintains optimal chemical environment for platelets. It buffers pH changes, maintains carbon dioxide partial pressure, and prevents direct contact between platelets and harsh storage conditions, thereby preserving platelet integrity and function
3Productivity
If conventional storage is used, then storage process is simple, but economic losses occur due to high expiration scrap rate of 10.3%
Solution Approach 1:
By changing storage parameters to controlled partial pressure of carbon dioxide (30-50 mmHg) and temperature (20-24°C), the patent extends platelet shelf life from 5 days to 7 days or more, which increases platelet utilization efficiency and reduces expiration-related waste
Solution Approach 2:
The patent implements preliminary optimization of storage conditions before platelet expiration occurs. By pre-establishing the optimal gas environment and temperature control, the system proactively prevents platelet deterioration and extends usable storage time, thereby improving productivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively alleviates PSLs, prolongs post-transfusion survival, protects hemostatic function, and extends the storage life of platelets to 7 days from the conventional 5 days.
Implementation Method 1
adjusting a partial pressure of carbon dioxide in a suspension medium containing cells, tissues or organs to be stored to 30-50 mmHg
Implementation Method 2
regulating (e.g., increasing) the partial pressure of carbon dioxide in the suspension medium containing the platelet concentrate to be stored to 30-50 mmHg can effectively alleviate platelet CD62P expression, CD42b shedding, PS eversion, glucose consumption, lactic acid accumulation and reduction in ATP production
Data Source
AI summary
The disclosure discloses an anti-lesion storage method and storage system for cells, tissues and organs, and relates to the technical field of storage of cells, tissues or organs. The method includes: adjusting a partial pressure of carbon dioxide in a suspension medium containing cells, tissues or organs to be stored to 30-50 mmHg. The inventors found that the regulation of the partial pressure of carbon dioxide in the suspension medium is advantageous for improving anti-lesions of cells, tissues or organs. In particular, the regulation of the partial pressure of carbon dioxide in the suspension medium can prolong the in vivo survival of platelets after transfusion of a platelet concentrate and protect the hemostatic function of platelets whiling effectively alleviating PSLs.