PmDR739 Molecular Marker Primer for Powdery Mildew-Resistant Wheat
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Solution Overview
Problem
Existing wheat powdery mildew resistance genes are prone to pathogen race specialization, leading to resistance loss, and there is a need for new resistance genes from Triticum dicoccoides to enhance genetic diversity and breeding efficiency.
Innovation Solution
A primer for amplifying a closely linked molecular marker of the powdery mildew resistance gene PmDR739 of Triticum dicoccoides, utilizing a codominant SSR marker HENU686, to accurately detect and select for powdery mildew-resistant wheat varieties through PCR and electrophoresis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional chemical control methods are used to control wheat powdery mildew, then disease control effectiveness is achieved, but safety and economic costs deteriorate
Solution Approach 1:
The patent replaces chemical control methods with biological breeding methods. By developing molecular markers (HENU686-F and HENU686-R primers) that can identify powdery mildew resistance genes in Triticum dicoccoides, the invention enables marker-assisted selection breeding, substituting chemical interventions with genetically-based resistance solutions that are safer and more economically sustainable
Solution Approach 2:
The patent changes the approach from external chemical control to internal genetic resistance by identifying and utilizing specific molecular markers linked to resistance genes. This parameter change from chemical to genetic control mechanisms achieves disease resistance through plant自身的 genetic properties rather than external chemicals
2Productivity
If single resistance genes are utilized for breeding, then breeding efficiency is improved, but pathogen race specialization accelerates and resistance is lost
Solution Approach 1:
The patent segments the resistance breeding approach by utilizing multiple independently inherited resistance genes from Triticum dicoccoides rather than relying on single major genes. This segmentation allows for pyramiding multiple resistance genes, which slows pathogen adaptation and maintains durable resistance while still achieving efficient breeding through molecular marker guidance
Solution Approach 2:
The patent applies the composite principle by combining multiple resistance genes from Triticum dicoccoides into wheat varieties. This genetic composite approach, guided by molecular markers, creates varieties with stacked resistance mechanisms that are more durable against pathogen race specialization while maintaining breeding efficiency
3Measurement precision
If molecular marker detection is implemented for precise selection, then selection precision is improved, but detection complexity and cost increase
Solution Approach 1:
The patent extracts specific molecular markers (HENU686-F and HENU686-R primers) that are closely linked to powdery mildew resistance genes from the complex genome of Triticum dicoccoides. By isolating and utilizing these specific marker loci, the invention achieves precise selection without requiring complex whole-genome analysis, thereby reducing detection complexity while maintaining high selection precision
Solution Approach 2:
The patent uses DNA copying through PCR amplification with specific primers to detect the presence of resistance-associated molecular markers. This copying approach allows for simple, rapid, and cost-effective detection compared to more complex methods, achieving high precision selection through amplified DNA fragments rather than complex genomic analysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and precise screening of high-quality powdery mildew-resistant wheat varieties, reducing environmental influence and production costs while improving selection efficiency and quality.
Implementation Method 1
performing polymerase chain reaction (PCR) amplification on the genomic DNA of the wheat sample to be detected by utilizing a molecular marker primer to obtain an amplification product
Implementation Method 2
performing electrophoresis and detection on the amplification product
Data Source
AI summary
Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR739 of Triticum dicoccoides. The molecular marker primer includes a forward primer HENU686-F and a reverse primer HENU686-R, where the nucleotide sequence of the forward primer HENU686-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU686-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR739, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.