PmDR754 Molecular Marker Primer for Wheat Powdery Mildew Breeding

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current methods for wheat powdery mildew resistance are inadequate, as many resistance genes have lost effectiveness due to pathogen mutation, and there is a need for new resistance genes and molecular markers to enhance breeding efficiency and stability.

Innovation Solution

Development of a primer for amplifying a molecular marker of the powdery mildew resistance gene PmDR754 from Triticum dicoccon Schrank, utilizing primers HENU654-F and HENU654-R, for PCR-based detection and marker-assisted selection breeding.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional wheat powdery mildew resistance genes (Pm1a, Pm8, Pm12) are used for breeding, then initial resistance to powdery mildew is achieved, but resistance is gradually lost due to pathogen mutation

Engineering Contradiction:

Improveresistance stabilityVSAvoidresistance duration

Core Design Contradiction:

ReliabilityVSDuration of action of moving object

Solution Approach 1:

The patent applies preliminary action by developing molecular markers (HENU654-F and HENU654-R primers) that can detect the PmDR754 resistance gene before breeding operations. This allows breeders to identify and select plants carrying the resistance gene in advance, ensuring reliable and durable resistance against powdery mildew before pathogen mutation can compromise effectiveness.

Inventive Principle:

Principle #10Preliminary action

2Reliability

If resistance genes from related species (Pm2, Pm8, Pm21, Pm58) are introduced through backcrossing, then wheat resistance is improved, but breeding complexity and time requirements increase

Engineering Contradiction:

Improveresistance effectivenessVSAvoidbreeding time

Core Design Contradiction:

ReliabilityVSLoss of time

Solution Approach 1:

The patent uses molecular markers as an intermediary tool to facilitate the introduction of resistance genes from related species. The primers HENU654-F and HENU654-R serve as mediators that enable direct detection and selection of resistance genes, eliminating the need for time-consuming multi-generational backcrossing and phenotypic selection, thus reducing breeding time while maintaining resistance effectiveness.

Inventive Principle:

Principle #24Intermediary (Mediator)

3Reliability

If Pm12 gene is used for high resistance, then powdery mildew resistance is significantly improved, but yield and quality traits deteriorate

Engineering Contradiction:

Improveresistance levelVSAvoidbreeding feasibility

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent applies local quality by enabling precise detection and selection of the PmDR754 resistance gene using molecular markers, allowing breeders to specifically target and maintain the resistance trait while independently managing and preserving yield and quality traits. This localized selection approach avoids the negative pleiotropic effects associated with Pm12, achieving high resistance without compromising breeding feasibility.

Inventive Principle:

Principle #3Local quality

4Object-affected harmful factors

If chemical control and existing resistance genes are used for powdery mildew management, then disease control is achieved, but environmental pollution and pathogen resistance development occur

Engineering Contradiction:

Improvedisease control effectivenessVSAvoidenvironmental pollution

Core Design Contradiction:

Object-affected harmful factorsVSObject-generated harmful factors

Solution Approach 1:

The patent replaces chemical control methods with a molecular-based detection and selection system. The primers HENU654-F and HENU654-R enable genetic identification and selection of resistant plants, substituting chemical interventions with a biological/molecular approach that achieves disease control effectiveness without generating environmental pollution or contributing to pathogen resistance development.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables rapid and precise identification of wheat varieties resistant to powdery mildew, improving breeding efficiency and reducing costs while ensuring high-quality resistance, unaffected by environmental factors.

Implementation Method 1

a primer for amplifying a molecular marker of powdery mildew resistance gene PmDR754 of Triticum dicoccon Schrank

Methodology Applied

Scientific EffectPCR amplification:

Data Source

PatentUS20250346964A1Primer for amplifying a molecular marker of powdery mildew resistance gene pmdr754 of triticum dicoccon schrank and application thereof

Publication Date: 2025.11.13 CROP RES INST SHANDONG ACAD OF AGRI SCI

AI summary

The disclosure discloses a primer for amplifying a molecular marker of powdery mildew resistance gene PmDR754 of Triticum dicoccon Schrank, where the molecular marker primer includes a forward primer HENU765-F and a reverse primer HENU765-R; the nucleotide sequence of the forward primer HENU765-F is shown in SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU765-R is shown in SEQ ID NO:2. The molecular marker primer provided by the disclosure is applied to detection and identification of gene PmDR754 and auxiliary identification of a wheat powdery mildew resistance trait, and molecular marker-assisted selection breeding.