PmDR803 Molecular Marker Primer for Wheat Powdery Mildew Breeding

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Solution Overview

Problem

Current methods for controlling wheat powdery mildew rely heavily on chemical treatments, and there is a need for more effective and sustainable breeding strategies using resistance genes, particularly from Triticum carthlicum, to enhance genetic diversity and resistance in wheat varieties.

Innovation Solution

Development of a primer for amplifying a closely linked molecular marker of the powdery mildew resistance gene PmDR803 in Triticum carthlicum, utilizing a forward primer HENU629-F and reverse primer HENU629-R, to facilitate molecular marker-assisted selection breeding and accurate detection of the gene.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical control methods are used to control wheat powdery mildew, then disease control effectiveness is improved, but environmental pollution and production costs increase

Engineering Contradiction:

Improvedisease control effectivenessVSAvoidenvironmental pollution

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent replaces chemical control methods with molecular marker-assisted selection breeding, substituting a mechanical/chemical system with a biological/genetic system. By using the HENU629 molecular marker to identify and breed plants carrying the PmDR803 resistance gene, the invention eliminates the need for chemical fungicides while achieving durable disease resistance through genetic improvement

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the fundamental parameter of disease control from external chemical application to internal genetic resistance. By detecting the presence of the PmDR803 gene through molecular markers, the breeding process selects for plants with inherent resistance capabilities, transforming the control mechanism from reactive chemical treatment to proactive genetic selection

Inventive Principle:

Principle #35Parameter changes

2Adaptability or versatility

If traditional breeding methods are used to develop resistant wheat varieties, then genetic diversity is improved, but breeding time and complexity increase

Engineering Contradiction:

Improvegenetic diversityVSAvoidbreeding time

Core Design Contradiction:

Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by developing and validating the HENU629 molecular marker in advance to specifically detect the PmDR803 resistance gene. This pre-established marker system allows breeders to quickly screen large populations for resistance gene presence without time-consuming phenotypic testing, significantly accelerating the breeding process while maintaining genetic diversity

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent introduces the HENU629 molecular marker as an intermediary between the resistance gene and the breeder's selection process. This molecular marker serves as a detectable proxy for the PmDR803 gene, enabling indirect selection that is both rapid and accurate, thereby reducing breeding time while preserving genetic diversity through marker-assisted selection

Inventive Principle:

Principle #24Intermediary (Mediator)

3Measurement precision

If molecular marker detection is used to identify resistance genes, then selection precision is improved, but detection complexity increases

Engineering Contradiction:

Improveselection precisionVSAvoiddetection complexity

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent extracts the specific genetic sequence information of the PmDR803 resistance gene and uses it to design the HENU629 molecular marker. By isolating and targeting only the relevant genetic region, the detection method achieves high precision for identifying resistance gene carriers while keeping the molecular biology techniques used relatively simple and accessible to breeding programs

Inventive Principle:

Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables rapid and precise identification of wheat varieties carrying the PmDR803 gene, improving selection efficiency and quality of powdery mildew-resistant wheat strains while reducing environmental impact and production costs.

Implementation Method 1

performing polymerase chain reaction (PCR) amplification on the genomic DNA of the wheat sample to be detected by utilizing a molecular marker primer to obtain an amplification product

Methodology Applied

Scientific EffectPolymerase chain reaction (PCR):

Data Source

PatentUS20250346966A1PRIMER FOR AMPLIFYING CLOSELY LINKED MOLECULAR MARKER OF POWDERY MILDEW RESISTANCE GENE PmDR803 OF TRITICUM CARTHLICUM AND APPLICATION THEREOF

Publication Date: 2025.11.13 CROP RES INST SHANDONG ACAD OF AGRI SCI

AI summary

Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR803 of Triticum carthlicum. The molecular marker primer includes a forward primer HENU629-F and a reverse primer HENU629-R, where the nucleotide sequence of the forward primer HENU629-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU629-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR803, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.