Polarized Bio-Chip Layout for Fluorescent Crosstalk Isolation
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Solution Overview
Problem
The reduction in array size on bio-chips leads to crosstalk between neighboring wells, making it difficult to detect individual fluorescent signals accurately, resulting in inaccurate analytical results.
Innovation Solution
A bio-chip with a polarizing array and a depletion light source, utilizing organic photoelectric conversion elements and polarizing elements to selectively collect signals from specific reaction sites, and a bio-detection system that includes a filter to reject excitation light, thereby improving signal detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the array size on the bio-chip is reduced to increase density, then the throughput and cost-effectiveness are improved, but crosstalk between neighboring wells occurs and detection precision deteriorates
Solution Approach 1:
The patent divides each pixel unit into multiple sub-polarizing units with different polarizing angles (e.g., 0°, 45°, 90°, 135°). Each sub-polarizing unit corresponds to a specific reaction site, allowing independent detection of fluorescent signals from different wells within the same pixel unit. This segmentation enables precise signal isolation despite reduced array spacing.
Solution Approach 2:
Different regions (sub-polarizing units) within each pixel unit are assigned different polarizing properties. By matching the polarizing angle of each sub-polarizing unit with the orientation of fluorescent molecules in corresponding reaction sites, the system achieves location-specific signal detection. This local differentiation allows neighboring wells to be distinguished even when physically close.
2Ease of manufacture
If the array size on the bio-chip is reduced to increase density, then the cost is reduced, but crosstalk between neighboring wells occurs
Solution Approach 1:
The patent introduces polarizing elements as intermediary components between the fluorescent molecules and the photodetector. These polarizing elements act as selective mediators that allow only light with specific polarization orientations to pass through to the corresponding sub-polarizing units. This intermediary mechanism effectively blocks crosstalk signals from neighboring wells while permitting target signals to pass.
3Use of energy by moving object
If organic photoelectric conversion elements with thickness greater than 500 nm are used, then the absorption of excitation light is improved, but the device complexity increases
Solution Approach 1:
The patent changes the thickness parameter of the organic photoelectric conversion element to greater than 500 nm. This parameter adjustment enhances the absorption of excitation light by the fluorescent molecules, improving signal generation efficiency. The increased thickness ensures sufficient light interaction volume while maintaining compatibility with the polarizing array structure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively reduces crosstalk and enhances the precision of fluorescent signal detection, ensuring accurate analytical results by selectively collecting signals from individual reaction sites.
Implementation Method 1
the bio-chip includes organic photoelectric conversion element, which may absorb short wavelength light, so that the organic photoelectric conversion element may be used as a filter that can partially reject the excitation light
Implementation Method 2
a polarizing element configured to polarize the depletion light, which may help to selectively collect a signal from a specific reaction site
Implementation Method 3
The fluorescent molecules may be excited by an excitation light with a shorter wavelength and generate an emission light with a longer wavelength toward the photodetector
Data Source
AI summary
A bio-chip is provided. The bio-chip includes a substrate and a first organic photoelectric conversion element disposed on the substrate. The first organic photoelectric conversion element defines pixel units. The bio-chip also includes a polarizing array disposed on the first organic photoelectric conversion element. The polarizing array includes polarizing sets, each polarizing set corresponds to one pixel unit and has sub-polarizing units that have different polarizing angles. The bio-chip further includes reaction sites disposed on the polarizing array. Each reaction site corresponds to one sub-polarizing unit.