Polyamine Production via Acid Hydrolysis and Bacillus Fermentation
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Solution Overview
Problem
Current methods for obtaining biogenic polyamines like histamine, putrescine, spermidine, and cadaverine from protein materials are inefficient in terms of concentration and time, with variable incubation times and unclear yield in existing processes.
Innovation Solution
A method involving hydrolysis of protein material with 18% sulphuric acid followed by fermentation using Bacillus subtilis at controlled temperatures and pH, and subsequent purification through filtering and spray drying to obtain high concentrations of these polyamines.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If traditional fermentation methods are used to obtain biogenic polyamines from protein materials, then the process is simple to operate, but the concentration of polyamines obtained is low and the time required is long
Solution Approach 1:
The patent applies preliminary action by performing acid hydrolysis on the protein material before fermentation. This pre-treatment step breaks down proteins into amino acids and peptides, making them more accessible to microbial degradation during subsequent fermentation. The hydrolysis step creates a substrate that is optimized for the microbial conversion to polyamines, thereby increasing both concentration and reducing time without complicating the overall process flow
Solution Approach 2:
The patent applies parameter changes by optimizing specific fermentation conditions including maintaining pH between 4.5-6.5, temperature at 25-37°C, and using specific microorganism strains. These parameter optimizations enhance the efficiency of polyamine production from amino acids, achieving higher concentrations in shorter times while maintaining operational simplicity through standardized control parameters
2Device complexity
If existing methods are used for obtaining polyamines, then the process can be performed with simple equipment, but the yield and concentration are not well-defined and variable
Solution Approach 1:
The patent applies feedback by implementing monitoring and control of key parameters during fermentation, including pH, temperature, and microbial activity. This feedback mechanism ensures consistent polyamine production by adjusting conditions to maintain optimal ranges, thereby achieving defined and reproducible yields without requiring complex equipment - only standard process control measures
Solution Approach 2:
The patent applies preliminary action by pre-characterizing the microbial strains and optimizing the substrate composition before large-scale fermentation. This preliminary work establishes standardized protocols that ensure consistent yields across different batches, reducing variability while maintaining equipment simplicity through standardized operating procedures
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the production of biogenic polyamines with high concentration and improved yield in a shorter timeframe compared to traditional methods, utilizing Bacillus subtilis to convert amino acids into polyamines effectively.
Implementation Method 1
performing a hydrolysis process
Implementation Method 2
fermenting wherein the microorganism Bacillus subtilis is used in order to convert the amino acids into these polyamines
Implementation Method 3
concentrating the obtained product by means of spray drying
Data Source
Figure 1
AI summary
A method for obtaining biogenic polyamines from a protein material with a protein richness of approximately 10% by weight, wherein the method includes the following steps: performing hydrolysis of a protein material with a protein richness of approximately 10%, followed by filtering to obtain a liquid permeate with a content of hydrolysed protein between 3 and 9%; ii. fermenting the permeate obtained in i. by adding the microorganism Bacillus subtilis at a temperature of 25° to 35°C and keeping the pH between 5 and 7; and iii. purifying by means of filtering to eliminate the insoluble microorganisms and concentrating the obtained product by means of spray drying.