Polybacterial Probiotic Preparation for Cholesterol Reduction
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Solution Overview
Problem
There is a need for a probiotic preparation that combines strains of lactic acid bacteria with specific health benefits such as high survival in the gastrointestinal tract, strong adhesion to the epithelial layer, antioxidant effects, cholesterol reduction capacity, and anti-inflammatory immune modulation, which are not effectively addressed by existing probiotic products.
Innovation Solution
A polybacterial preparation comprising Lactobacillus gasseri 7/12, Lactobacillus plantarum F12, and Lactobacillus helveticus A1 strains, identified through precise DNA-based methods and phenotypic identification, which exhibit antioxidant activity, strong adhesion, cholesterol reduction, and anti-inflammatory effects, is developed for use in pharmaceutical and food industries.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing probiotic products use single or limited strains, then the product complexity is low, but the comprehensive health benefits (antioxidant, cholesterol reduction, immune modulation) are insufficient
Solution Approach 1:
The patent combines three different Lactobacillus strains (L. plantarum F12, L. gasseri 7/12, and L. helveticus A1) into a single polybacterial preparation. Each strain contributes specific functional properties: L. plantarum F12 provides antioxidant activity and adhesion, L. gasseri 7/12 provides cholesterol reduction capacity, and L. helveticus A1 provides anti-inflammatory effects. This merging of multiple strains resolves the contradiction by achieving comprehensive health benefits while managing the complexity through synergistic combination.
Solution Approach 2:
The polybacterial preparation is designed to perform multiple health functions simultaneously: antioxidant protection, cholesterol reduction, immune modulation, and anti-inflammatory effects. By incorporating strains with complementary functionalities, the single preparation product achieves universal applicability for preventing multiple conditions (hypertension, arteriosclerosis, hypercholesterolemia, intestinal inflammation), thus resolving the versatility-complexity contradiction.
2Adaptability or versatility
If probiotic strains are selected for multiple specific functions (adhesion, antioxidant, cholesterol reduction), then the health benefits are comprehensive, but the strain selection and identification process becomes more complex
Solution Approach 1:
The patent replaces traditional phenotypic identification methods with DNA-based identification methods for strain characterization. This substitution enables more precise and reliable identification of the three Lactobacillus strains, allowing researchers to accurately select strains with specific functional properties (adhesion, antioxidant activity, cholesterol reduction) without being limited by the complexity and subjectivity of conventional identification techniques.
3Adaptability or versatility
If the preparation targets multiple diseases (hypertension, arteriosclerosis, hypercholesterolemia, inflammation), then the preventive coverage is broad, but the product formulation becomes more complex
Solution Approach 1:
The polybacterial preparation is formulated as a universal probiotic product that simultaneously addresses multiple health conditions: hypertension (through ACE-inhibiting peptides from L. helveticus A1), arteriosclerosis and hypercholesterolemia (through cholesterol reduction by L. gasseri 7/12), and intestinal inflammation (through anti-inflammatory effects of all strains). This multi-functional formulation resolves the contradiction by providing broad disease prevention coverage within a single integrated product rather than requiring separate formulations for each condition.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polybacterial preparation effectively reduces cholesterol levels, exhibits significant antioxidant activity, and modulates the immune response, providing a comprehensive health benefit for preventing hypertension, arteriosclerosis, hypercholesterolemia, and intestinal inflammation.
Implementation Method 1
reduction of cholesterol concentration due to hydrolysis of bile salts and direct absorption of cholesterol
Implementation Method 2
antioxidant effect... Some microorganisms possess antioxidant enzymes such as SOD (Superoxide dismutases), catalase enzymes, glutathione reductase enzymes and others
Implementation Method 3
Stimulation of the immune response through immunomodulation and enhancement of inherited immunity... Probiotic strains that lead to stimulation of the induction of IL-10 are suitable for people suffering from inflammatory disease of the colon
Implementation Method 4
strong adhesion to the epithelial layer... Representatives of both genuses can colonize the mucosa layer of the colon
Data Source
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AI summary
This invention relates to polybacterial probiotic preparation, which includes new strains of lactic acid bacteria Lactobacillus gasseri 7/12 NBIMCC No. 8720, Lactobacillus plantarum F12 NBIMCC No 8722 and Lactobacillus helveticus Al, NBIMCC No 8721 and has the following set of properties : antioxidant, hypocholesterolemic effect, anti-inflammatory immunomodulating effect, ability to release bioactive peptides that inhibit angiotensin-converting enzyme (ACE), which is responsible for the high blood pressure. The object of invention is also the described probiotic strains of lactic acid bacteria and the developed bacterial products with useful properties. The incorporated probiotic strain Lactobacillus gasseri 7/12 NBIMCC No. 8720, has a number of properties: strong adhesion to the epithelial layer of the colon, the ability for reduction of cholesterol and anti-inflammatory effects by reducing the levels of proinflammatory cytokine interleukin-8. The invention relates to the use of strains of lactic acid bacteria and bacterial agents in the production of starter cultures, food additives, food, functional products and pharmaceutical preparations intended to influence the health of humans and animals.