Polycaprolactone Nonwoven Sheet for Stable Stem Cell Retention
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Solution Overview
Problem
Existing tissue regeneration promoting materials face challenges in stably retaining mesenchymal stem cells due to their porous structure or polymer material powder composition, leading to instability and difficulty in retaining other stem cells.
Innovation Solution
A tissue regeneration promoting sheet composed of a nonwoven fabric made of polycaprolactone fibers with a specific diameter range, impregnated with a cell suspension solution, and reinforced by a biocompatible polymer nonwoven fabric to stabilize stem cell retention and promote tissue regeneration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If porous bodies or polymer material powder are used as tissue regeneration promoting materials, then the materials can be manufactured with existing techniques, but the stable retention of mesenchymal stem cells becomes difficult
Solution Approach 1:
The patent changes the physical parameters of the fiber structure, specifically using ultra-fine fibers with a diameter of 1 μm or less (preferably 0.1 to 1 μm). This parameter change creates a fiber mat structure that effectively retains mesenchymal stem cells while maintaining manufacturability through existing nonwoven fabric production techniques
Solution Approach 2:
The patent uses composite materials by combining ultra-fine fibers with specific basis weights (1 to 10 g/m²) to create a nonwoven fabric structure that provides both mechanical stability and effective cell retention. The combination of fiber diameter and basis weight creates an optimal structure for stem cell retention
2Reliability
If the fiber diameter is reduced to improve stem cell retention, then the retention effectiveness increases, but the fabric strength and handling properties deteriorate
Solution Approach 1:
The patent optimizes the fiber diameter parameter to 1 μm or less (preferably 0.1 to 1 μm) which maximizes stem cell retention effectiveness. This ultra-fine fiber diameter creates sufficient surface area and interaction points for cell adhesion while maintaining fabric integrity through controlled basis weight
Solution Approach 2:
The patent applies different quality characteristics to different aspects of the nonwoven fabric: ultra-fine fibers (0.1 to 1 μm) provide local cell retention functionality, while the overall fabric structure with controlled basis weight (1 to 10 g/m²) provides mechanical strength and handling properties
3Strength
If the basis weight is increased to improve fabric strength, then the handling properties improve, but the stem cell retention capability deteriorates
Solution Approach 1:
The patent optimizes the basis weight parameter to a specific range of 1 to 10 g/m² (preferably 1 to 3 g/m²). This parameter optimization achieves the optimal balance where the fabric has sufficient mechanical strength for handling while maintaining ultra-fine fiber structure that effectively retains mesenchymal stem cells
Data Source
AI summary
A tissue regeneration promoting sheet (10) includes a cell retention sheet (12) that is a nonwoven fabric, and the nonwoven fabric contains fibers based on polycaprolactone as a main component and is configured to be impregnated with a cell suspension solution containing stem cells. The fiber diameter of the fibers forming the nonwoven fabric may be in a range of 300 nm to 2 μm. The basis weight of the nonwoven fabric may be in a range of 1 g/m2 to 3 g/m2. The thickness of the nonwoven fabric may be larger than or equal to 300 μm. The tissue regeneration promoting sheet (10) may further include a reinforcement sheet (14) laminated on the nonwoven fabric and configured to be impregnated with the cell suspension solution.