Polyepoxy Raw-Material Composition for Uniform Chromatography Pores
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Solution Overview
Problem
Chromatographic microspheres face issues such as low processing capacity, long process cycles, low yield of effective active biomacromolecules, poor inter-batch stability, and high production costs, while monolithic columns suffer from uneven internal structures and poor repeatability, leading to lower separation efficiency.
Innovation Solution
A raw material composition for chromatographic materials comprising 20%-70% of a substance with a polyepoxy group, 30%-80% of a pore-forming agent, and 0.1‰-1‰ of a catalyst, which includes polyepoxy glycidyl ether substances, is used to create a chromatographic material with controlled pore channels and even distribution, allowing for high ligand utilization and efficient biomacromolecule separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chromatographic microspheres are used for biomacromolecule purification, then separation function is achieved, but processing capacity is low and process cycle is long
Solution Approach 1:
The patent employs a monolithic column structure with controlled porosity and interconnected pore channels, replacing traditional packed microsphere beds. This continuous porous network enables rapid biomacromolecule diffusion throughout the column without dead zones, significantly increasing processing capacity while reducing the time required for complete diffusion and separation.
Solution Approach 2:
The monolithic column incorporates segmented or modular design elements that facilitate optimized flow distribution and reduced channeling effects. This segmentation approach eliminates the vortex formation and dead pores inherent in packed microsphere systems, thereby improving both productivity and reducing process cycle time.
2Area of stationary object
If chromatographic microspheres with porous structure are used, then surface area is increased, but vortex effect and shear force increase leading to low yield
Solution Approach 1:
The monolithic column provides a continuous porous matrix with uniform pore distribution and interconnected channels. This structure delivers high effective surface area for ligand attachment while maintaining laminar flow patterns that minimize vortex formation and shear stress on biomacromolecules, thereby preserving yield.
Solution Approach 2:
The patent optimizes the local pore structure and ligand distribution within the monolithic column to ensure uniform flow characteristics throughout. This localized optimization prevents dead pores and vortex zones, reducing harmful shear forces while maintaining high surface area availability for separation.
3Reliability
If chromatographic microspheres are used, then separation function is achieved, but inter-batch stability is poor and production cost is high
Solution Approach 1:
The monolithic column fabrication process utilizes controlled polymerization parameters to achieve consistent pore structure, ligand density, and mechanical properties across batches. This parameter control approach, combined with simplified single-step manufacturing, reduces production variability and cost compared to multi-step microsphere synthesis.
Solution Approach 2:
The patent employs composite monolithic materials combining polymer matrices with functional ligands in a single integrated structure. This composite approach simplifies manufacturing relative to assembling multiple microsphere components, improving inter-batch stability while reducing production complexity and cost.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution provides chromatographic materials with adjustable and evenly distributed pore channels, reducing retention time, minimizing vortex effects, and enhancing yield and stability, resulting in a lower production cost and improved separation efficiency.
Implementation Method 1
0.1‰-1‰ of a catalyst C
Data Source
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AI summary
A raw material composition for a chromatography material, a chromatography material, a preparation method therefor and the use thereof, a monolithic column, and a chromatopile. The raw material composition for a chromatography material, based on 100% of the total weight of a raw material A and a pore-forming agent B, comprises the following components: 20-70% of the raw material A; 30-80% the pore-forming agent B; and 0.1-1‰ of a catalyst C, wherein the raw material A comprises a substance containing multiple epoxy groups. The chromatography material prepared from the chromatography material composition can solve the current issue of the use of a chromatography microsphere filler in biological macromolecules. The chromatography material has the characteristics of uniform internal structure, good preparation reproducibility, high toughness, ability to modify a functional ligand and high hydrophilicity, and is more suitable for separation and purification of biomolecules.