Polyisocyanopeptide Hydrogel Crosslinking for Mechanical Stability

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Solution Overview

Problem

Existing polyisocyanopeptide hydrogels exhibit temperature-dependent mechanical properties, making it challenging to maintain consistent mechanical properties over time without altering their architecture.

Innovation Solution

A method for selectively crosslinking polyisocyanopeptide hydrogels within the bundles rather than randomly throughout the network, using a crosslinking unit with a specific size to form predominantly intrabundular crosslinks, thereby stabilizing the architecture and mechanical properties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If common crosslinking approaches are used to stabilize mechanical properties, then the hydrogel becomes more stable over time, but the network architecture changes and mechanical properties are altered

Engineering Contradiction:
Improvestability of mechanical propertiesVSAvoidnetwork architecture
Core Design Contradiction:
Stability of the object's compositionVSShape

Solution Approach 1:

The patent applies local quality by directing crosslinks specifically inside the bundles rather than randomly throughout the network. The crosslinking is localized to specific regions (inside bundles) to achieve stabilization without altering the overall network architecture. This selective placement of crosslinks within bundles maintains the external network structure while providing internal stabilization.

Inventive Principle:
Principle #3Local quality

2Strength

If the hydrogel is heated above LCST to form gel, then mechanical properties are achieved, but temperature dependence causes mechanical properties to change over time

Engineering Contradiction:
Improvemechanical propertiesVSAvoidtemperature dependence
Core Design Contradiction:
StrengthVSTemperature

Solution Approach 1:

The patent applies preliminary action by performing crosslinking inside the bundles before the hydrogel is subjected to temperature variations during use. This pre-established crosslinking network provides a stable framework that resists temperature-induced changes, locking in the mechanical properties formed during gelation and maintaining them across temperature ranges.

Inventive Principle:
Principle #10Preliminary action

3Stability of the object's composition

If crosslinks are placed randomly in the network, then stabilization is achieved, but the unique bundled architecture is lost

Engineering Contradiction:
Improvestabilization of propertiesVSAvoidbundled architecture
Core Design Contradiction:
Stability of the object's compositionVSShape

Solution Approach 1:

The patent applies local quality by directing crosslinks specifically inside the bundles rather than randomly throughout the network. The crosslinking is localized to specific regions (inside bundles) to achieve stabilization without altering the overall network architecture. This selective placement of crosslinks within bundles maintains the external network structure while providing internal stabilization.

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach stabilizes the mechanical properties of the hydrogel, reducing temperature dependence and maintaining the unique mechanical properties of the polyisocyanopeptide hydrogels, ensuring consistency over a large temperature range.

Implementation Method 1

FG is a functional moiety that can be covalently coupled to the complementary functional moiety F1 or F2 of the crosslinking unit (B)

Methodology Applied
Scientific EffectCovalent bonding: Chemical Bonding

Implementation Method 2

A solution of these polymers in water will show Lower Critical Solution Temperature (LCST) behavior; above the LCST a hydrogel is formed by formation of a branched network of entangled semi-flexible bundles, a consequence of entropic desolvation of the ethylene glycol moieties on the polymers

Methodology Applied
Scientific EffectLCST behavior: Phase Change

Data Source

PatentEP3548107B1Biomimetic networks comprising polyisocyanopeptide hydrogels
Publication Date: 2023.06.07 STICHTING RADBOUD UNIVERSITEIT
  • EP3548107B1 patent drawingFigure 1~2
  • EP3548107B1 patent drawingFigure 3~4
  • EP3548107B1 patent drawingFigure 5~6

AI summary

A polymer hydrogel comprising a polymer formed by the crosslinking reaction of a polymeric unit A according to formula (I), with a crosslinking unit B according to formula (II), and water, wherein n = 100 - 10,000, preferable 250 - 2500, more preferable 500 - 1500; m = independently 2-10, preferably 3 or 4; FG is a functional moiety that can be covalently coupled to the complementary functional moiety F1 or F2 of the crosslinking unit (B); k = 0.01 - 0.05; h = 0, 1 or 2; the spacer is an organic moiety, having a main chain comprising at least two functional moieties F1 and F2, wherein the length of the crosslinker in the extended conformation as determined by molecular modeling (including spacer and functional groups F1 and F2) is between 2.5 and 12 nm, or wherein the length is between 20 and 80 atoms.