Polylysine Conjugate Stabilizes Unstable Molecules in Solution
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Solution Overview
Problem
Current methods for stabilizing macromolecules in solution are inadequate, as they either require cumbersome processes, are not suitable for therapeutic or nutritional use, or pose environmental and health risks, and fail to maintain stability and activity over extended periods at temperatures above 0°C.
Innovation Solution
A non-covalent molecular complex comprising a polylysine (PLL) conjugate and a molecule unstable in solution, where the PLL conjugate improves the solubility and stability of the molecule through non-covalent bonding, allowing for extended storage and use in therapeutic, cosmetic, or diagnostic applications without significant loss of activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If conventional stabilization methods (micro-encapsulation, nanoparticles) are used, then stability is improved, but device complexity and manufacturing complexity increase
Solution Approach 1:
The invention extracts the essential stabilizing function from complex systems like micro-encapsulation and nanoparticles, achieving stabilization through a simple PLL-based approach without requiring elaborate structural designs
Solution Approach 2:
The patent uses readily available PLL material that can be easily synthesized and modified, replacing expensive and complex nanoparticle systems with a more accessible polymer-based solution
2Stability of the object's composition
If conventional stabilization methods are used, then stability is improved, but ease of manufacture deteriorates
Solution Approach 1:
The invention extracts the essential stabilizing function from complex manufacturing processes, achieving stabilization through simple PLL conjugation that can be performed using standard biochemical techniques
Solution Approach 2:
The patent uses readily available PLL material that can be easily synthesized and modified, replacing expensive and complex nanoparticle systems with a more accessible polymer-based solution
3Stability of the object's composition
If temperature stabilization at -80°C is used, then stability is improved, but energy consumption increases
Solution Approach 1:
The invention changes the stabilization parameter from temperature-dependent (requiring -80°C) to chemical-structure-dependent (PLL-conjugated molecules), allowing stable storage at ambient temperatures without energy-intensive cooling
Solution Approach 2:
The patent converts the inherent instability of hydrophobic molecules in aqueous solutions into a benefit by using PLL's hydrophilic nature and ability to form stable conjugates, transforming a problem into a solution that eliminates the need for extreme temperature control
4Stability of the object's composition
If conventional stabilization methods are used, then stability is improved, but harmful factors increase
Solution Approach 1:
The patent converts the inherent instability of hydrophobic molecules in aqueous solutions into a benefit by using PLL's hydrophilic nature and ability to form stable conjugates, transforming a problem into a solution that eliminates the need for extreme temperature control
Solution Approach 2:
The invention changes the stabilization parameter from temperature-dependent (requiring -80°C) to chemical-structure-dependent (PLL-conjugated molecules), allowing stable storage at ambient temperatures without energy-intensive cooling
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The PLL-based molecular complex effectively stabilizes and maintains the activity of unstable molecules in solution for several days, months, or years at ambient temperature, enabling safe and effective use in various applications without immunogenicity, toxicity, or environmental harm.
Implementation Method 1
A non-covalent molecular complex comprising a polylysine (PLL) conjugate and a molecule unstable in solution, where the PLL conjugate improves the solubility and stability of the molecule through non-covalent bonding
Data Source
AI summary
The invention relates to a molecular complex comprising at least one polylysine conjugate (PLL), comprising a main PLL straight chain and at least one molecule F having an average molecular weight of between 50 daltons and 1000 daltons that is covalently bonded to said main chain, and at least one molecule M that is unstable in solution, the conjugate(s) and the molecule(s) M being bonded by means of a non-covalent bond. The invention also relates to a composition comprising a complex of this kind, to a method for obtaining said composition and use thereof, and to the use of one or more PLL-based conjugates for improving the hydrophilicity, the effectiveness, and the activity of a molecule that is unstable in solution, over a time period that is compatible with the use of said molecule. The invention also relates to a method for identifying a PLL-based conjugate or a combination of a plurality of PLL-based conjugates that makes it possible to improve the hydrophilicity, the effectiveness, and the activity of a molecule that is unstable in solution, and to a kit for implementing said method.