Polymannan Extract Formulation for Cancer Immunomodulation
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Solution Overview
Problem
Current treatments for various cancers such as leukemias, lymphomas, prostate cancer, breast cancer, and colon cancer, as well as immune-related disorders, lack effective immunomodulatory agents that can significantly stimulate the immune system and induce apoptosis in cancer cells.
Innovation Solution
A method for preparing a fine powder of polymannan extract from Aloe species, involving steps like dissolving freeze-dried Aloe powder in deionized water, adding an alcoholic solvent, centrifuging, and lyophilizing to produce a sterile injectable formulation that increases natural killer cells and induces apoptosis in cancer cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current treatments for cancers and immune disorders are used, then existing therapeutic options are available, but they lack effective immunomodulatory activity and ability to induce apoptosis in cancer cells
Solution Approach 1:
The patent extracts and isolates specific polysaccharide fractions (acemannan and aloeride) from Aloe vera gel through a multi-step process involving precipitation with ethanol, centrifugation, and fractionation. This extraction isolates the immunomodulatory components from the whole plant material, concentrating the active principles that stimulate immune response and induce apoptosis in cancer cells.
Solution Approach 2:
The patent utilizes changes in solvent polarity and molecular weight parameters to separate and isolate different polysaccharide fractions. By adjusting ethanol concentration and performing size-exclusion chromatography, the patent isolates high molecular weight polysaccharides (acemannan) and lower molecular weight fractions (aloe ride) with distinct immunomodulatory properties, optimizing their therapeutic effectiveness.
2Reliability
If polysaccharide fractions are isolated from Aloe vera, then immunostimulatory activity is achieved, but the complexity of the isolation and purification process increases
Solution Approach 1:
The patent divides the polysaccharide isolation process into distinct sequential stages: initial precipitation with ethanol to remove proteins and contaminants, centrifugation to separate solids from liquids, and fractionation to isolate specific molecular weight ranges. This segmentation simplifies each individual step while achieving the overall goal of purifying immunostimulatory polysaccharides.
Solution Approach 2:
The patent employs ethanol precipitation as a multi-functional step that simultaneously removes proteins, contaminants, and lower molecular weight substances while concentrating the high molecular weight immunostimulatory polysaccharides. This universal approach eliminates the need for multiple specialized purification techniques, reducing process complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polymannan extract formulation shows a 75-80% increase in natural killer cells and elevated caspase 3 levels, indicating effective immunomodulation and apoptosis in cancer cells, thereby treating the mentioned cancers and immune disorders.
Implementation Method 1
The polymannan extract formulation shows a 75-80% increase in natural killer cells and elevated caspase 3 levels, indicating effective immunomodulation and apoptosis in cancer cells
Implementation Method 2
The polymannan extract formulation shows a 75-80% increase in natural killer cells and elevated caspase 3 levels, indicating effective immunomodulation and apoptosis in cancer cells
Data Source
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Figure 4A~4B
AI summary
The present invention describes a method for preparing a polymannan extract from freeze- dried aloe powder. The polymannan extract of the present invention is further used to formulate a sterile injectable formulation for the treatment of one or more cancers, leukemias and lymphomas, prostate cancer, breast cancer, and colon cancer, immune diseases, particularly immune related neoplasms, acquired immune deficiency syndrome, and hepatitis C.