Polymer Culture Medium Cation Removal for Cell Suspension
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Solution Overview
Problem
Current methods for suspending animal and plant cell cultures face challenges such as low solubility of polymer compounds with anionic functional groups in water, leading to increased viscosity and difficulties in cell dispersion and recovery, which affects cell function and scalability.
Innovation Solution
A method involving the removal of divalent metal cations from polymer compounds like deacylated gellan gum using chelating agents or ion exchange resins, allowing these compounds to dissolve in water at room temperature and form a medium composition that uniformly disperses cells without increasing viscosity, facilitating suspension culture.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If polymer compounds with anionic functional groups are used to suspend cells in culture medium, then cell suspension and dispersion are improved, but solubility in water is poor leading to increased viscosity
Solution Approach 1:
The patent uses divalent metal cations (Ca2+, Mg2+) as intermediary substances that bridge the anionic functional groups of polymer compounds. These cations form ion complexes with the polymers, enabling suspension without requiring high polymer concentration, thus improving solubility while maintaining cell dispersion capability
Solution Approach 2:
The patent changes the chemical parameters of the system by introducing divalent metal cations that alter the electrochemical properties of the polymer compounds. This parameter change (adding cations) transforms the solubility characteristics and viscosity behavior of the polymer solutions
2Ease of operation
If polymer compounds with anionic functional groups are used for cell suspension, then cell dispersion is improved, but viscosity of the medium increases
Solution Approach 1:
Divalent metal cations serve as mediators that enable effective cell dispersion through electrostatic interaction with anionic polymers at low concentrations. This intermediary mechanism achieves dispersion functionality without the high viscosity associated with high polymer concentrations
Solution Approach 2:
The patent changes the physical parameter of viscosity by introducing cation-polymer complexes. The presence of divalent cations alters the hydrodynamic properties and intermolecular interactions, resulting in lower viscosity while maintaining dispersion effectiveness
3Quantity of substance
If heat treatment is used to dissolve polymer compounds in water, then solubility is improved, but cell function may be affected
Solution Approach 1:
Divalent metal cations act as intermediaries that enable polymer dissolution at room temperature through complex formation. This eliminates the need for heat treatment, thereby preserving cell function and viability while achieving complete polymer solubility
Solution Approach 2:
The patent performs preliminary action by pre-mixing divalent metal cations with polymer compounds before adding to cell culture medium. This preliminary complex formation occurs under mild conditions, ensuring polymer solubility is achieved without subsequent heat treatment that could harm cells
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enhances the solubility of polymer compounds in water, enabling efficient and scalable suspension culture of cells without heat treatment, maintaining cell function and simplifying recovery processes.
Implementation Method 1
The polymer compound having an anionic functional group is mixed with a chelating agent or a cation exchanger to remove a divalent metal cation
Implementation Method 2
The polymer compound having an anionic functional group is mixed with a chelating agent or a cation exchanger to remove a divalent metal cation
Implementation Method 3
mixing a powder obtained by drying a polymer compound having an anionic functional group, which shows improved solubility in water by removing a divalent metal cation(s), and a medium
Data Source
AI summary
The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.