Polymer-Coated Nanoparticle Complex for Biological Target Isolation
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Solution Overview
Problem
In biological samples with complex components, the matrix effect causes nanoparticles to aggregate, reducing detection sensitivity and making it difficult to isolate target materials efficiently.
Innovation Solution
A nanoparticle complex is developed with a receptor bonded to polymer-coated nanoparticles, specifically using polymers like polydopamine and polyethylene glycol to prevent matrix effects, allowing for enhanced dispersion and efficient isolation of target materials.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If nanoparticles are used in biological samples, then detection sensitivity can be improved, but particle aggregation occurs due to matrix effect reducing detection sensitivity
Solution Approach 1:
The patent introduces a polymer coating layer as an intermediary substance between the nanoparticle core and the biological sample environment. This polymer layer acts as a mediator that prevents direct interaction between the nanoparticle surface and the complex biological matrix, thereby preventing aggregation while maintaining detection sensitivity. The polymer coating serves as a protective interface that resolves the contradiction between utilizing nanoparticles for sensitive detection and maintaining their stable dispersed state.
Solution Approach 2:
The patent modifies the surface properties of nanoparticles by coating them with polymers, thereby changing physical and chemical parameters such as surface charge, hydrophilicity, and steric hindrance. These parameter changes prevent the matrix effect from causing aggregation, allowing nanoparticles to maintain stable dispersion in complex biological samples while retaining their detection capabilities.
2Productivity
If nanoparticles are used to isolate target materials, then isolation efficiency can be improved, but particle aggregation due to matrix effect makes it difficult to isolate target materials efficiently
Solution Approach 1:
The polymer coating serves as an intermediary that enables efficient isolation by preventing aggregation during the isolation process. The coated nanoparticles maintain stable dispersion while interacting with target materials, allowing efficient separation and isolation without the particles clumping together, which would reduce isolation efficiency.
3Stability of the object's composition
If polymer coating is applied to nanoparticles, then particle dispersion is enhanced and matrix effect is suppressed, but device complexity increases
Solution Approach 1:
The patent employs thin polymer film coatings on nanoparticle surfaces to achieve dispersion enhancement and matrix effect suppression. These thin flexible polymer layers provide steric stabilization and prevent aggregation without significantly increasing the overall size or complexity of the nanoparticle system. The thin film approach maintains simplicity while achieving the desired stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The nanoparticle complex significantly increases detection sensitivity and isolation efficiency by preventing particle aggregation in biological samples, enabling accurate detection and diagnosis of diseases using biomarkers.
Implementation Method 1
a polymer material that can coat nanoparticles by a self-polymerization reaction
Implementation Method 2
can bind to nanoparticles having a thiol group
Implementation Method 3
the dispersed state of the nanoparticle complex can be effectively maintained by reducing the matrix effect
Implementation Method 4
a receptor for detecting or isolating a target material can be bound to the macromolecule to specifically bind to the target material
Data Source
AI summary
The present invention relates to a nanoparticle complex for isolation of a biological target and a preparation method therefor and, more specifically, to a nanoparticle complex in which a receptor is conjugated to a polymer-coated nanoparticle, a preparation method therefor, and a use thereof.