Organic Polymer Core-Shell Particles for pH-Stable Bioseparations
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Solution Overview
Problem
Current superficially porous chromatographic materials lack pH stability in high acid or base environments, have limited pore size options, and exhibit a wide particle size distribution, which hampers their performance in bio-separations.
Innovation Solution
Development of organic polymer core-shell particles with a non-porous core and a porous shell, featuring a narrow particle size distribution and broad flexibility in pore diameter selection, using organic polymers with C—C, C—O, and C—N covalent bonds, formed via radical or condensation polymerization, and surface modification for enhanced stability and separation efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If silica or inorganic hybrid materials are used for core-shell particles, then pH stability is improved relative to traditional silica materials, but further improvements in pH stability are needed particularly for bio-separations requiring high concentration of base or acid
Solution Approach 1:
The patent employs organic polymer core-shell particles where both the core and shell are composed of organic polymer materials. This composite organic polymer structure provides superior chemical stability and pH resistance compared to inorganic hybrid materials, enabling the particles to withstand extreme pH conditions required for bio-separation applications while maintaining structural integrity and functional performance
2Reliability
If current superficially porous chromatographic materials are used, then a core-shell structure is achieved, but the pore size selection is limited and flexibility is reduced
Solution Approach 1:
The patent systematically varies the pore size parameter of the organic polymer shell across a broad range from 60 Å to 3000 Å by controlling polymerization conditions and monomer selection. This parameter change approach enables flexible adaptation to different chromatographic applications, including bio-separations, while maintaining the beneficial core-shell structural configuration
3Ease of manufacture
If traditional superficially porous materials with thin porous layers are used, then particle formation is achieved, but the particle size distribution is polydisperse and broad
Solution Approach 1:
The patent creates a core-shell structure where the inner core region and outer shell region have distinct properties. The core provides structural stability while the shell provides controlled porosity. This local differentiation of properties enables precise control over particle size distribution and pore characteristics, achieving monodisperse particles with narrow size distribution while maintaining ease of manufacture
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The organic polymer core-shell particles demonstrate improved pH stability from pH 1 to 12, narrow particle size distribution, and the ability to form a broad array of pore sizes, enhancing mass transfer and separation efficiency for both small molecules and large biologics.
Implementation Method 1
a porous organic polymer shell, in which the porous organic polymer shell has a pore size ranging from 60 Å to 3000 Å
Data Source
AI summary
In various embodiments, the present disclosure pertains to organic polymer core-shell particles that comprise a non-porous organic polymer core (i.e., having a pore volume of less than 0.1 cc/g) and a porous organic polymer shell (i.e., having a pore volume of greater than 0.1 cc/g), in which the porous organic polymer shell has a pore size ranging from 100 Å to 3000 Å. In some embodiments, the present disclosure pertains to chromatographic separation devices that comprise such organic polymer core-shell particles. In some embodiments, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such organic polymer core-shell particles and (b) flowing a mobile phase through the column.