Polymer Dye Immobilization via Boronic Ester Bonds for Stable H2O2 Detection
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Solution Overview
Problem
Existing methods for detecting hydrogen peroxide in biological samples are hindered by the adsorption of dyes onto impurities, leading to unstable detection due to separation and elution of the dye from the detection surface.
Innovation Solution
A polymer dye is developed, comprising specific repeating units that include a light-absorbing material with an ionic or nonionic functional group, which is immobilized on a solid surface using boronic ester bonds to prevent separation and elution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a functional dye is used to detect hydrogen peroxide, then detection sensitivity is improved, but the dye is adsorbed on impurities and separated/eluted from the device surface, causing unstable detection
Solution Approach 1:
The patent introduces a solid support (resin particles, glass surface, or polymer membrane) as an intermediary carrier to immobilize the functional dye. This mediator prevents direct contact between the dye and impurities in the sample, eliminating adsorption issues while maintaining detection sensitivity. The dye is covalently bonded to the solid support, creating a stable detection system that resolves the contradiction between sensitivity and stability.
2Ease of manufacture
If a dye is simply immobilized on resin particle surfaces by boronic ester bonds, then immobilization is achieved, but the dye still separates and elutes due to adsorption on impurities
Solution Approach 1:
The patent creates a composite structure combining the solid support (resin particles or other substrates), the immobilization layer (boronic ester bonds or other coupling agents), and the functional dye. This multi-component composite system provides both easy immobilization and stable detection by distributing the dye within a protective matrix that prevents impurity adsorption while maintaining optical detection capabilities.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polymer dye enables stable and sensitive detection of hydrogen peroxide even in the presence of impurities, as it remains effectively bonded to the surface, allowing for accurate quantification of hydrogen peroxide.
Implementation Method 1
immobilized on a solid surface using boronic ester bonds to prevent separation and elution
Implementation Method 2
Dye is a light absorbing material which contains an ionic or nonionic functional group and in which the maximum value of a molar extinction coefficient at a wavelength of 400 nm to 700 nm is 10^4 M^-1cm^-1 or more
Data Source
AI summary
A polymer dye includes at least one repeating unit represented by structural formula 7 and at least one selected from the group consisting of repeating units represented by structural formulas 2 and 3:Hydrogen peroxide present in or generated through enzymatic reaction in a biological sample can be detected by reacting with the polymer dye.