Polymer-Polymer Exosome Matrix to Prevent Agglomeration

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Solution Overview

Problem

Exosomes derived from mesenchymal stem cells face challenges in maintaining their structural integrity and stability, particularly due to agglomeration, which reduces their efficacy when particle size exceeds 200 nm.

Innovation Solution

A composition comprising a polymer-polymer complex of natural or synthetic sulfated polymers crosslinked with cationic polyamines is used to stabilize exosomes, preventing agglomeration and maintaining their integrity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If exosomes are cryogenically frozen to prevent agglomeration, then exosome stability is improved, but exosome functionality deteriorates due to loss of structural integrity upon thawing

Engineering Contradiction:

Improveexosome stabilityVSAvoidexosome functionality

Core Design Contradiction:

Stability of the object's compositionVSReliability

Solution Approach 1:

The patent introduces a polymer-polymer complex as an intermediary stabilizing agent between exosomes and the environment. This complex, formed by crosslinking anionic polymers with cationic polyamines, acts as a protective mediator that prevents direct contact between exosomes and harmful conditions, thereby maintaining both stability and functionality without requiring cryogenic freezing.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent employs a composite polymer-polymer complex structure combining anionic polymers (such as carboxymethyl cellulose, chitosan, or hyaluronic acid) with cationic polyamines through crosslinking. This composite material provides enhanced stabilizing properties that protect exosomes from agglomeration while preserving their structural integrity and biological activity upon thawing.

Inventive Principle:

Principle #40Composite materials

2Shape

If exosomes are kept in liquid form to maintain integrity, then exosome structure is preserved, but exosomes aggregate and lose efficacy when stored for extended periods

Engineering Contradiction:

Improveexosome structureVSAvoidstorage duration

Core Design Contradiction:

ShapeVSDuration of action of stationary object

Solution Approach 1:

The patent applies preliminary action by pre-forming a polymer-polymer complex that is ready to stabilize exosomes before storage. The crosslinked structure is prepared in advance to immediately prevent agglomeration when exosomes are introduced, enabling extended storage while maintaining structural integrity and preventing efficacy loss.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The patent utilizes parameter changes by modifying the physical-chemical environment through the polymer complex, which alters the colloidal stability parameters of exosome suspensions. This changes the interaction parameters between exosomes, preventing aggregation while allowing extended storage at physiological temperatures without compromising structure or function.

Inventive Principle:

Principle #35Parameter changes

3Reliability

If exosomes are used immediately after thawing to maintain efficacy, then exosome functionality is preserved, but time loss occurs due to immediate usage requirement

Engineering Contradiction:

Improveexosome efficacyVSAvoidusage time window

Core Design Contradiction:

ReliabilityVSLoss of time

Solution Approach 1:

The polymer-polymer complex is prepared in advance as a stabilizing formulation that can be stored with exosomes before use. This preliminary preparation eliminates the need for immediate usage after thawing, as the stabilizing complex is already in place to maintain efficacy during extended storage periods, thereby reducing time loss.

Inventive Principle:

Principle #10Preliminary action

4Reliability

If polymer concentration is increased to enhance stabilization, then exosome protection is improved, but formulation complexity increases

Engineering Contradiction:

Improveexosome protectionVSAvoidformulation complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent optimizes the concentration parameters of the polymer and polyamine components to achieve effective stabilization at relatively low concentrations. By carefully adjusting these parameters, the formulation maintains exosome protection while minimizing formulation complexity and avoiding excessive polymer loads that would complicate the system.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The polymer-polymer complex effectively stabilizes exosomes, preserving their structure and functionality for extended periods, enhancing their therapeutic effects in wound healing and tissue regeneration.

Implementation Method 1

a polymer-polymer complex including one or more natural or synthetic sulfated polymers crosslinked with one or more crosslinking agent cationic polyamines

Methodology Applied

Scientific EffectElectrostatic interaction: Electrostatics

Data Source

PatentUS20260000607A1Polymer-polymer Matrix for the Stabilization of Exosomes

Publication Date: 2026.01.01 GHALILI BABAK

AI summary

The present disclosure relates to exosomes and compositions including one or more natural or synthetic sulfated polymers crosslinked with one or more crosslinking agent cationic polyamines as well as methods of use and methods of making of them.