Polymer-Filled Chromatography Resin for High-Flow Biomolecule Separation
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Solution Overview
Problem
Current methods for purifying viruses, proteins conjugated to large particles, and other large biomolecules from smaller impurities are inefficient, requiring large columns, low flow rates, and limited sample loads, and have limited selectivity, making them costly and impractical for dense biomolecules.
Innovation Solution
A method of preparing a chromatography resin by adding a melted polymer to a chromatography resin, allowing it to absorb into the resin's pores, atomizing the mixture, and collecting polymer-filled beads to create a resin with multimodal properties that maintains selectivity and binding capacity, using a scalable and environmentally friendly process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If size exclusion chromatography is used to purify large biomolecules, then separation based on size is achieved, but large columns are required, flow rates must be low, and sample loads are limited
Solution Approach 1:
The patent uses porous polymer particles filled within the pores of chromatography resin beads. The porous structure provides size-based exclusion while maintaining open flow paths, enabling high flow rates without sacrificing separation resolution. The filler particles create a size exclusion effect that allows large biomolecules to pass through while retaining smaller impurities.
Solution Approach 2:
The invention creates a composite chromatography resin by combining base resin beads with porous polymer filler particles. This composite structure integrates the size exclusion properties of the filler with the mechanical stability and flow characteristics of the base resin, achieving both high resolution and high productivity simultaneously.
2Productivity
If ion exchange or hydrophobic interaction chromatography is used, then purification is achieved, but selectivity is limited
Solution Approach 1:
The porous polymer filler particles provide size-based separation that is independent of the chemical properties of the base resin. This adds a size exclusion dimension to the existing ion exchange or hydrophobic interaction mechanisms, enhancing overall selectivity without reducing purification efficiency.
3Adaptability or versatility
If centrifugation is used for dense biomolecules, then separation from smaller impurities is achieved, but application is limited to relatively dense biomolecules
Solution Approach 1:
The porous filler particles create a size exclusion effect that separates biomolecules based on their hydrodynamic radius rather than density. This allows the method to purify less dense biomolecules like viruses and protein-nanoparticle conjugates that cannot be effectively separated by centrifugation, while maintaining high purification effectiveness.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces polymer-filled chromatography resin beads that effectively separate target molecules from contaminants without significantly affecting resin selectivity or binding capacity, enabling efficient purification of protein-nanoparticle conjugates and viruses.
Implementation Method 1
adding a chromatography resin to a solution comprising a melted polymer while stirring to allow the melted polymer to absorb or otherwise enter the pores of the chromatography resin
Implementation Method 2
The solution is then atomized and insoluble porous polymer-filled chromatography resin beads are collected in a water bath
Data Source
AI summary
Methods of preparing polymer-filled chromatography resin and their uses are provided.