Polymeric Dye Cyclodextrin Quenching Reduction

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing dimeric and polymeric fluorescent or colored dyes suffer from intramolecular fluorescence quenching, limiting their brightness and effectiveness in analytical methods, despite efforts to enhance signal intensity.

Innovation Solution

Incorporating cyclodextrins into polymeric dyes to prevent or reduce intramolecular quenching, resulting in compositions that are intensely colored or fluorescent and exhibit higher molar brightness.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Illumination intensity

If dimeric and polymeric compounds comprising two or more fluorescent or colored moieties are prepared to increase brightness, then the signal intensity should increase, but intramolecular fluorescence quenching occurs and the desired brightness increase is not achieved

Engineering Contradiction:

Improvefluorescence brightnessVSAvoidfluorescence quenching

Core Design Contradiction:

Illumination intensityVSLoss of energy

Solution Approach 1:

The polymeric dye is segmented into multiple units where each unit comprises a fluorescent or colored moiety separated by spacer groups. This segmentation prevents intramolecular quenching by maintaining appropriate distances between chromophores while still achieving enhanced brightness through the cumulative effect of multiple moieties.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

Spacer groups act as intermediaries between the fluorescent or colored moieties in the polymeric structure. These spacers prevent direct interaction that would cause quenching while allowing the moieties to contribute to the overall signal intensity, thus resolving the contradiction between increasing brightness and avoiding energy loss.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Adaptability or versatility

If water soluble dyes are used for biomolecule analysis in aqueous environment, then the dyes can be applied to biological samples, but the molar brightness is insufficient for highly sensitive detection

Engineering Contradiction:

Improvewater solubilityVSAvoidmolar brightness

Core Design Contradiction:

Adaptability or versatilityVSIllumination intensity

Solution Approach 1:

The invention creates composite polymeric dye structures that combine multiple fluorescent or colored moieties with spacer groups and water-solubilizing elements. This composite approach maintains water solubility for biomolecule analysis while achieving high molar brightness through the cooperative effect of multiple chromophores without intramolecular quenching.

Inventive Principle:

Principle #40Composite materials

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The use of cyclodextrins in polymeric dyes enhances their molar brightness, allowing for more effective detection of analytes, including biomolecules, by reducing fluorescence quenching and improving visibility without prior illumination or chemical activation.

Implementation Method 1

dimeric and polymeric fluorescent or colored dyes

Methodology Applied

Scientific EffectFluorescence: Fluorescence

Implementation Method 2

the cyclodextrin in the composition binds to one or more dye moieties in the polymeric dye

Methodology Applied

Scientific EffectCyclodextrin binding: Absorption (physical)

Data Source

PatentEP3455299B1Compositions comprising a polymeric dye and a cyclodextrin and uses thereof

Publication Date: 2024.01.17 SONY GROUP CORP

Figure 1
Figure 2
Figure 3

AI summary

Compositions comprising a polymeric dye and a cyclodextrin are provided. Methods associated with preparation and use of such compounds, for example in methods for detection of any analyte molecule, are also provided.