Polymeric Dyes Deep UV Excitation Background Fluorescence

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Solution Overview

Problem

Current fluorescent dyes lack effective deep ultraviolet excitation spectra, limiting their application in biochemistry and medicine due to insufficient penetration and high background fluorescence, which hampers precise molecular recognition and analysis.

Innovation Solution

Development of water-soluble polymeric dyes with a light harvesting multichromophore and conjugation-modifying repeat units that absorb in the deep ultraviolet range, combined with a signaling chromophore for enhanced energy transfer and reduced cross-source excitation, allowing for deeper tissue penetration and improved signal-to-noise ratios.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Illumination intensity

If conventional fluorescent dyes are used, then they provide sufficient fluorescence emission, but they lack deep ultraviolet excitation spectra resulting in high background fluorescence and insufficient tissue penetration

Engineering Contradiction:

Improvedeep ultraviolet excitation spectrumVSAvoidbackground fluorescence

Core Design Contradiction:

Illumination intensityVSObject-affected harmful factors

Solution Approach 1:

The patent changes the excitation wavelength parameter by designing polymeric dyes with deep ultraviolet absorption maxima (280-350 nm), fundamentally shifting from conventional visible light excitation. This parameter change enables deeper tissue penetration and reduces background fluorescence interference

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The invention uses composite polymeric dye structures combining multiple chromophores with specific conjugation patterns. These composite materials exhibit deep ultraviolet absorption properties that individual conventional dyes lack, achieving both deep tissue penetration and low background fluorescence

Inventive Principle:

Principle #40Composite materials

2Measurement precision

If conventional fluorescent dyes are used, then they provide adequate signal detection, but they suffer from insufficient signal-to-noise ratios due to high background fluorescence

Engineering Contradiction:

Improvesignal-to-noise ratioVSAvoidbackground fluorescence

Core Design Contradiction:

Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

By changing the excitation wavelength to deep ultraviolet range (280-350 nm), the patent exploits the fact that biological tissues have minimal endogenous fluorescence in this range, thereby dramatically improving signal-to-noise ratio and measurement precision

Inventive Principle:

Principle #35Parameter changes

3Length of moving object

If conventional fluorescent dyes are used, then they provide sufficient fluorescence for detection, but they lack deep tissue penetration capability

Engineering Contradiction:

Improvetissue penetration depthVSAvoidexcitation spectrum

Core Design Contradiction:

Length of moving objectVSIllumination intensity

Solution Approach 1:

The patent shifts the excitation spectrum parameter to deep ultraviolet wavelengths (280-350 nm), which have higher energy and better penetration characteristics through biological tissues compared to conventional visible light excitation, enabling deeper imaging

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The polymeric dyes provide a deep ultraviolet excitation spectrum, minimizing background fluorescence and enhancing signal-to-noise ratios, enabling more precise molecular recognition and analysis in biochemistry and medicine.

Implementation Method 1

water solvated polymeric dyes having a deep ultraviolet excitation spectrum

Methodology Applied

Scientific EffectAbsorption: Absorption (EM radiation)

Implementation Method 2

Fluorescent dyes are compounds which, when irradiated with light of a wavelength which they absorb, emit light of a (usually) different wavelength

Methodology Applied

Scientific EffectFluorescence: Fluorescence

Implementation Method 3

a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith

Methodology Applied

Scientific EffectEnergy transfer:

Data Source

PatentUS20240141175A1Deep Ultraviolet-Excitable Water-Solvated Polymeric Dyes

Publication Date: 2024.05.02 BECTON DICKINSON & CO

AI summary

Water solvated polymeric dyes having a deep ultraviolet excitation spectrum are provided. The subject polymeric dyes include a light harvesting multichromophore having conjugation-modifying repeat units incorporated into the polymer backbone to provide segments of π-conjugated co-monomers having limited π-conjugation between segments. Polymeric tandem dyes are also provided that further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject water solvated polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.