Polymeric Fluorescent Dyes With Magnesium for Quenching Control

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Solution Overview

Problem

Existing dimeric and polymeric fluorescent or colored dyes do not achieve the desired increase in brightness due to intramolecular fluorescence quenching, limiting their effectiveness in analytical methods requiring highly sensitive detection.

Innovation Solution

The use of polymeric dyes with specific structures and the presence of magnesium salts enhances fluorescence or color intensity by minimizing quenching effects, allowing for brighter and more sensitive detection of analyte molecules.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Illumination intensity

If dimeric and polymeric dyes are used to increase brightness, then the signal intensity should improve, but intramolecular fluorescence quenching occurs reducing the actual brightness

Engineering Contradiction:

Improvefluorescence brightnessVSAvoidintramolecular fluorescence quenching

Core Design Contradiction:

Illumination intensityVSObject-generated harmful factors

Solution Approach 1:

The patent divides the polymeric dye structure into separated fluorescent moieties connected by spacing groups, preventing close proximity interactions that cause quenching while maintaining the multiplicative brightness effect

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

Spacing groups act as intermediary elements between fluorescent moieties, maintaining optimal distances that prevent quenching while allowing energy retention and signal amplification

Inventive Principle:

Principle #24Intermediary (Mediator)

2Ease of operation

If conventional fluorescent dyes are used in aqueous environments, then water solubility is maintained, but signal-to-noise ratio is insufficient for highly sensitive detection

Engineering Contradiction:

Improvewater solubilityVSAvoidsignal-to-noise ratio

Core Design Contradiction:

Ease of operationVSMeasurement precision

Solution Approach 1:

The patent creates composite fluorescent polymers combining multiple fluorescent moieties with spacing groups and water-solubilizing groups, achieving both high brightness and water solubility simultaneously

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

Multiple fluorescent moieties are merged into a single polymeric structure to amplify the signal, while water-solubilizing groups ensure the composite remains soluble in aqueous detection environments

Inventive Principle:

Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method provides intensely colored or fluorescent polymeric dyes with increased molar brightness, enabling improved detection of biomolecules by enhancing signal-to-noise ratio and spatial-temporal distribution analysis.

Implementation Method 1

Fluorescent and/or colored dyes are known to be particularly suitable for applications in which a highly sensitive detection reagent is desirable

Methodology Applied

Scientific EffectFluorescence: Fluorescence

Implementation Method 2

as a result of intramolecular fluorescence quenching, the known dimeric and polymeric dyes have not achieved the desired increase in brightness

Methodology Applied

Scientific EffectFluorescence quenching: Fluorescence

Data Source

PatentEP4137818B1Use of divalent metals for enhancement of fluorescent signals

Publication Date: 2025.12.10 SONY GROUP CORP

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AI summary

Compositions comprising fluorescent or colored dyes and methods of using the same in combination with divalent or trivalent metal salts to analyze samples are disclosed.