Polymethine Fluorescent Dyes for Organ Function Measurement
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Solution Overview
Problem
Current methods for determining organ function, particularly liver and kidney function, face challenges such as inadequate precision, reliance on indirect indicators, and logistical difficulties with existing tracer-based tests, which can be unreliable in pathophysiological conditions like sepsis.
Innovation Solution
The use of polymethine fluorescent dyes with a fluorescence emission maximum of less than or equal to 820 nm as markers for data acquisition, allowing for more precise imaging and measurement of organ function through radiative excitation and fluorescence detection, providing better representation of perfusion and excretory capacity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If radioactive tracers or substrates are used for dynamic liver tests, then direct information about actual functional state is obtained, but logistical effort and laboratory procedures become complex and difficult to implement in routine practice
Solution Approach 1:
The patent replaces radioactive tracers with fluorescent dyes that can be detected by fluorescence spectroscopy. This substitution eliminates the need for complex radioactive material handling, special licensing, and dedicated laboratory facilities, while maintaining the ability to perform dynamic functional tests in routine clinical settings
Solution Approach 2:
The patent changes the detection parameter from radioactive decay to fluorescence emission. By using fluorescent dyes with specific emission wavelengths, the method achieves direct measurement of organ function through non-invasive or minimally invasive fluorescence detection, avoiding the logistical complexities of radioactive tracer administration and measurement
2Length of moving object
If conventional fluorescence dyes with emission maximum above 820 nm are used, then deep tissue penetration is achieved, but measurement precision and signal resolution are reduced
Solution Approach 1:
The patent optimizes the fluorescence emission wavelength parameter to be at or below 820 nm. This parameter change achieves an optimal balance between tissue penetration depth and signal resolution, allowing for precise measurement of organ function while maintaining adequate penetration through biological tissues
3Ease of operation
If static enzyme measurements are used to assess liver cell death, then simple measurement procedures are maintained, but reliability decreases in chronic hepatitis or cirrhosis where enzyme release decreases
Solution Approach 1:
The patent replaces static enzyme measurements with dynamic fluorescence-based functional tests. By administering fluorescent substrates and measuring their metabolism or excretion in real-time, the method provides reliable assessment of actual organ function rather than relying on static enzyme levels that may be depleted in chronic disease
Solution Approach 2:
The patent employs dynamic measurement over time rather than single-point static measurement. By tracking the time-course of fluorescent substrate metabolism or dye clearance, the method captures functional capacity that reflects current organ status, providing reliable results even when static enzyme levels are low or nonspecific
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables accurate assessment of organ function, including liver and kidney performance, with improved diagnostic accuracy and prognostic value, particularly in conditions like sepsis, by decoupling uptake and excretion processes and providing a direct measure of excretory capacity.
Implementation Method 1
one or more polymethine fluorescent dyes with a fluorescence emission maximum measured in ethanol of less than or equal to 820 nm
Data Source
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AI summary
The invention relates to a method for acquiring data in order to determine an organ function, in particular the liver or kidney function, comprising one or more polymethine fluorescent dyes having a fluorescence emission maximum measured in ethanol of less than or equal to 820 nm, and to the use of the dyes as markers for acquiring data in order to determine the organ function. The invention further relates to a kit for determining an organ function with a marker dye, and to a device for acquiring data in the method.