Polypeptide Platelet Aggregation Inducer for Aspirin-Resistant Testing
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Solution Overview
Problem
Conventional methods for measuring platelet aggregation capacity, such as using collagen of animal origin or artificially synthesized collagen, face challenges like low yield, high cost, and reduced effectiveness due to aspirin interference, which complicates the evaluation of platelet function.
Innovation Solution
A polypeptide represented by the formula (Pro-X-Gly)n, where X is Hyp and n ranges from 20 to 5,000, is polymerized in a solvent to create a platelet aggregation inducing substance with improved yield and cost efficiency, capable of effectively inducing platelet aggregation even in the presence of aspirin.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If artificially synthesized collagen is used to measure platelet aggregation capacity, then reproducibility and stability are improved, but yield decreases and cost increases
Solution Approach 1:
The patent changes the chemical structure parameters of the collagen molecule by using a specific polypeptide sequence (Pro-Hyp-Gly)n with controlled molecular weight and composition. This allows optimization of both reproducibility and yield by creating a simplified, standardized structure that can be efficiently synthesized without complex crosslinking procedures.
Solution Approach 2:
The patent extracts the essential functional elements of collagen required for platelet aggregation induction, separating them from the complex synthesis and crosslinking processes. By focusing only on the critical Pro-Hyp-Gly repeating unit, the patent eliminates unnecessary synthesis steps while maintaining biological activity, thereby improving yield and reducing cost.
2Reliability
If artificially synthesized collagen is used to measure platelet aggregation capacity, then stability is improved, but cost increases
Solution Approach 1:
The patent employs a simplified polypeptide structure that can be produced through straightforward polymerization processes, eliminating the need for expensive crosslinking reagents and complex purification steps. This approach uses simpler, more cost-effective materials and procedures while maintaining the stability and reproducibility needed for reliable platelet aggregation measurement.
3Reliability
If conventional collagen substances are used, then platelet aggregation can be induced, but effectiveness is reduced in the presence of aspirin
Solution Approach 1:
The patent creates a composite polypeptide structure combining specific amino acid sequences (Pro-Hyp-Gly)n with optimized molecular characteristics. This composite structure enhances the substance's ability to induce platelet aggregation by presenting multiple binding sites and structural features that resist inhibition by aspirin, thereby maintaining effectiveness in patients taking aspirin.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide solution provides sufficient platelet aggregation inducing activity, allowing for precise evaluation of platelet aggregation capacity without the need for crosslinking, thus overcoming the limitations of existing methods and maintaining effectiveness in the presence of aspirin.
Implementation Method 1
a polypeptide represented by formula (1) used in the invention can be obtained by polymerizing a trimer of - (Pro-Hyp-Gly) - in a solvent
Implementation Method 2
it is also necessary to perform crosslinking
Implementation Method 3
aggregation of platelets is induced by adding an aggregation inducing substance, such as collagen and the like, to platelet-rich plasma
Data Source
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AI summary
A platelet aggregation inducing substance containing as an active ingredient a polypeptide having a peptide fragment represented by formula (1) (component A) : -(Pro-X-Gly)n- (1) wherein X represents Pro or Hyp; and n represents an integer of from 20 to 5,000.