Polypeptide Barcode Libraries for Rapid Molecule Screening

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Solution Overview

Problem

Current high-throughput screening methods for small molecule compounds and biomacromolecules are inefficient and lack the ability to rapidly identify candidate molecules for disease diagnosis or treatment.

Innovation Solution

A polypeptide-encoded nucleic acid or small molecule compound library is developed, where each molecule is tagged with a unique polypeptide barcode, allowing for rapid identification and screening through mass spectrometry, enabling high-throughput analysis of large libraries.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional high-throughput screening methods are used, then screening capability is maintained, but screening efficiency and speed are insufficient

Engineering Contradiction:

Improvescreening efficiencyVSAvoidscreening time

Core Design Contradiction:

ProductivityVSLoss of time

Solution Approach 1:

The patent uses polypeptide barcode tags as information copies to represent nucleic acid molecules or small molecule compounds. Instead of directly analyzing each molecule, the barcode tags serve as identifiable copies that can be rapidly detected and correlated back to the original molecules, enabling high-throughput screening without analyzing every candidate individually

Inventive Principle:

Principle #26Copying

Solution Approach 2:

The patent replaces traditional mechanical/physical screening methods with a information-based identification system using polypeptide barcodes. The screening process is substituted with barcode reading and data analysis, significantly reducing the time and complexity of identifying candidate molecules from large libraries

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Quantity of substance

If large libraries of molecules are screened, then more candidates are available, but the complexity of identification increases

Engineering Contradiction:

Improvenumber of candidatesVSAvoididentification complexity

Core Design Contradiction:

Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent segments the identification complexity by assigning unique polypeptide barcode tags to individual molecules within large libraries. This segmentation allows each molecule to be independently identified through its barcode, simplifying the overall identification process even as the library size increases

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The polypeptide barcode tags act as intermediary elements between the nucleic acid molecules/small molecule compounds and the detection system. These barcodes serve as accessible intermediaries that can be easily read and correlated to the original molecules, reducing the direct complexity of identifying molecules from large libraries

Inventive Principle:

Principle #24Intermediary (Mediator)

Data Source

PatentUS20250340863A1Polypeptide-encoded library and screening method using same

Publication Date: 2025.11.06 WHOLESOME FUTURE LTD

AI summary

A polypeptide-encoded nucleic acid or small molecule compound library includes polypeptide barcode tags and nucleic acid molecules or small molecule compounds corresponding to the polypeptide barcode tags. Further provided is a polypeptide-encoded nucleic acid or small molecule compound library, the library comprising a plurality of beads, and each bead comprising a polypeptide barcode tag connected to the bead and a nucleic acid molecule or a small molecule compound corresponding to the polypeptide barcode tag. A method is for screening a target nucleic acid or small molecule via the polypeptide-encoded nucleic acid or small molecule compound library. A screened nucleic acid molecule or small molecule compound is identified by decoding a polypeptide barcode tag associated therewith.