Polypeptide Epoxy Group Removal for Trichothecene Detoxification

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Solution Overview

Problem

Current methods for detoxifying trichothecene mycotoxins are inefficient and require harsh conditions, and the mechanism of action by anaerobic bacteria is unclear, limiting practical industrial applications.

Innovation Solution

A polypeptide with epoxy group-removing catalytic activity that catalyzes a reaction between trichothecene mycotoxins and glutathione under mild conditions to produce a non-toxic glutathionylated derivative, utilizing specific amino acid sequences and nucleic acid molecules to encode and express the polypeptide.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If chemical synthesis under highly alkaline conditions is used to remove epoxy group, then epoxy group removal can be achieved, but reaction conditions become harsh and efficiency decreases

Engineering Contradiction:

Improvereaction conditionsVSAvoidreaction efficiency

Core Design Contradiction:

Ease of manufactureVSProductivity

Solution Approach 1:

The invention changes the reaction conditions from highly alkaline chemical synthesis to mild physiological conditions by introducing a polypeptide catalyst. The polypeptide enables epoxy group removal at neutral pH and moderate temperatures, fundamentally altering the reaction parameters to achieve both ease of manufacture and high productivity

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The invention replaces the chemical synthesis mechanism with a biological catalysis mechanism. Instead of using chemical reagents and harsh conditions, a polypeptide enzyme catalyzes the reaction between trichothecene mycotoxin and glutathione, substituting chemical mechanics with biological catalysis to achieve mild conditions and high efficiency simultaneously

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Productivity

If anaerobic bacteria are used for detoxification, then epoxy group removal can occur, but practical application is limited due to dependence on anaerobic conditions and unclear mechanism

Engineering Contradiction:

Improvedetoxification capabilityVSAvoidapplication conditions

Core Design Contradiction:

ProductivityVSAdaptability or versatility

Solution Approach 1:

The invention extracts the catalytic function from the complex anaerobic bacterial system and isolates it into a specific polypeptide sequence. By taking out only the essential catalytic component, the invention removes the dependency on anaerobic conditions and unclear bacterial mechanisms, enabling broad application under various conditions while maintaining high detoxification capability

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The invention introduces a polypeptide as an intermediary catalyst that mediates the reaction between trichothecene mycotoxin and glutathione. This polypeptide intermediary enables the detoxification process to proceed efficiently under controlled conditions without requiring anaerobic bacterial systems, thus improving adaptability while maintaining productivity

Inventive Principle:

Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The polypeptide effectively detoxifies trichothecene mycotoxins by converting them into harmless glutathionylated derivatives under mild conditions, reducing toxicity and improving industrial applicability.

Implementation Method 1

Methodology Applied

Scientific EffectCatalysis: Catalysis

Data Source

PatentUS20230151371A1Polypeptides having epoxy group-removing catalytic activity, nucleic acids encoding the polypeptides and use thereof

Publication Date: 2023.05.18 SHANDONG VEZYME BIOTECH CO LTD

AI summary

A polypeptide having epoxy group-removing catalytic activity with an amino acid sequence as set forth in SEQ ID NOs: 1-35, a nucleic acid molecule encoding the polypeptide, a nucleic acid construct comprising the nucleic acid, a pharmaceutical composition for detoxification and a food, beverage or feed composition comprising the polypeptide, and a host cell and an engineered microorganism into which the nucleic acid is introduced. Disclosed are a method for producing the polypeptide; and a method for catalyzing a reaction of removing an epoxy group of a trichothecene, a method for preventing cell poisoning or relieving cytotoxicity, a method for processing a food and beverage or feed composition, and a method for reducing or decreasing a toxin in a composition, all using the polypeptide. Further disclosed are a glutathionylated derivative, a method for evaluating the detoxification effect for a sample contaminated with a trichothecene using the glutathionylated derivative.