Polypeptide Magnetic Beads for LPS Removal
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Solution Overview
Problem
Current methods for removing lipopolysaccharides (LPS) from proteins, particularly in mass production using gram-negative bacteria like E. coli, face challenges due to the protein's charge state and hydrophobicity, leading to inefficient removal and increased production costs.
Innovation Solution
A composition and kit utilizing a polypeptide with a specific sequence formula, or its salt substitute, that exhibits high binding ability to LPS, allowing for effective removal through contact and isolation, with the polypeptide being reusable and having long-term storage stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If chromatography method is used to remove endotoxin, then endotoxin removal is achieved, but production cost increases and large amount of protein purification becomes difficult
Solution Approach 1:
The patent extracts the endotoxin removal function from complex chromatography systems and implements it through a simple magnetic bead-based system. The magnetic beads are functionalized with specific ligands that selectively bind endotoxins, allowing direct extraction and removal from protein solutions without requiring expensive chromatography equipment or large amounts of buffer materials.
Solution Approach 2:
The patent introduces magnetic beads as an intermediary carrier that mediates between the endotoxin target and the separation process. These beads are functionalized with endotoxin-binding ligands and can be easily manipulated using magnetic fields, serving as a versatile intermediary that simplifies the overall removal process while maintaining high efficiency.
2Object-affected harmful factors
If chromatography method is used to remove endotoxin, then endotoxin removal is achieved, but purification of large amount of protein becomes difficult
Solution Approach 1:
The patent changes the physical parameters of the removal system by using magnetic beads with high surface area to volume ratio and controlled surface chemistry. This allows the system to handle large volumes of protein solution efficiently, as the magnetic beads can be used in sufficient quantities and can be rapidly separated using magnetic fields, enabling scalable processing of large protein amounts.
3Object-affected harmful factors
If conventional methods are used to remove endotoxin from proteins, then removal is attempted, but removal is hindered by protein charge state and hydrophobicity
Solution Approach 1:
The patent uses magnetic beads that are functionalized with ligands specifically designed to copy or mimic the natural binding interactions that occur between endotoxins and their receptors. This allows the system to selectively bind endotoxins regardless of the protein's charge state or hydrophobicity, as the binding mechanism is based on specific molecular recognition rather than general electrostatic or hydrophobic interactions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide achieves high efficiency in removing LPS, maximizing protein purification and recovery rates, while being economical and maintaining effectiveness across multiple uses and long-term storage.
Implementation Method 1
a polypeptide or salt substitute thereof, having binding ability to lipopolysaccharide
Data Source
AI summary
The present invention relates to a composition and a kit for removing lipopolysaccharide (LPS), comprising a polypeptide having binding ability to lipopolysaccharide or a salt substitute thereof as an active ingredient, and a method for removing lipopolysaccharide. The polypeptide or a salt substitute thereof according to the present invention has very excellent binding ability with lipopolysaccharide, and the efficiency of removing lipopolysaccharide during a purification process in a protein production process using E. coli is high, such that the lipopolysaccharide removal efficiency can be maximized.