Polypeptide Binding Phosphatidylserine for Apoptotic Cell Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods lack effective and specific binding agents for phosphatidylserine, which is exposed on apoptotic and tumor cells, limiting diagnostic and therapeutic options for diseases associated with phosphatidylserine exposure.
Innovation Solution
A polypeptide with a specific amino acid sequence (designated as sequence number 1) is developed to bind selectively to phosphatidylserine, enabling the creation of compositions for detection, drug delivery, and treatment of tumorous diseases, as well as visualization of tumorous regions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods are used to detect phosphatidylserine, then detection can be performed, but the specificity and effectiveness are insufficient
Solution Approach 1:
The patent introduces a polypeptide as an intermediary binding agent that specifically recognizes phosphatidylserine on apoptotic and tumor cells. This polypeptide acts as a mediator between the detection system and the target cells, enabling highly specific detection through its unique binding capability to phosphatidylserine, thereby resolving the contradiction between detection precision and reliability
Solution Approach 2:
The invention changes the binding parameter by using a polypeptide with specific amino acid sequence that exhibits high affinity and specificity for phosphatidylserine. This parameter change in binding characteristics enables more effective and specific detection compared to conventional methods, simultaneously improving both measurement precision and reliability
2Adaptability or versatility
If non-specific binding agents are used, then broad coverage is achieved, but selectivity for phosphatidylserine is lost
Solution Approach 1:
The polypeptide is designed with specific local quality in its amino acid sequence that confers selective binding capability to phosphatidylserine. This localized specific binding property allows the agent to maintain broad applicability while achieving high selectivity for phosphatidylserine-containing cells, resolving the contradiction between adaptability and measurement precision
3Productivity
If drugs are delivered systemically, then all target cells are reached, but adverse effects on normal tissues increase
Solution Approach 1:
The polypeptide serves as a targeting intermediary that directs drug delivery specifically to phosphatidylserine-expressing tumor and apoptotic cells. By mediating between the drug and target cells, it enables selective delivery that maintains productivity while minimizing harmful effects on normal tissues through its specific binding recognition
Solution Approach 2:
The drug delivery system utilizes the local quality of phosphatidylserine expression on target cells to achieve site-specific drug delivery. The polypeptide-drug conjugate selectively binds to cells with phosphatidyls erine exposure, concentrating therapeutic effect at the target site while reducing systemic adverse effects, thus resolving the contradiction between productivity and harmful factors
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide effectively recognizes and binds to phosphatidylserine on apoptotic and tumor cells, facilitating their detection and targeted treatment, while reducing adverse effects on normal tissues by selective drug delivery.
Implementation Method 1
a polypeptide having an amino acid sequence designated as sequence number 1 and specifically binding to phosphatidylserine
Data Source
Figure 1A~1D
Figure 2A~2D
Figure 3A~3D
AI summary
The present invention relates to a polypeptide specifically bound to phosphatidylserine and use thereof, and more particularly to a polypeptide having an amino acid sequence designated as sequence number 1 and specifically bound to phosphatidylserine, a phosphatidylserine detecting composition containing the polypeptide as an active ingredient, a detecting method of phosphatidylserine by using polypeptide, a apoptotic cell detecting containing the polypeptide as an active ingredient, a drug delivery composition containing the polypeptide as an active ingredient, a composition for treatment and prevention of a tumorous disease, and a composition for visualization of a tumorous region. A polypeptide having an amino acid sequence designated sequence number 1 is specifically bound to phosphatidylserine. Therefore, the polypeptide of the present invention is useful for detecting phosphatidylserine, furthermore detecting apoptotic cells expressing phosphatidylserine on the surface of the cell and tumor cells, and also useful for visualization of apoptotic cells or tumor cells.