Polypeptide Production via Segmented Enzymatic Hydrolysis
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Solution Overview
Problem
Current nutritional supplements aimed at regulating blood glucose levels and reducing fat face challenges in quality control and product purity, particularly due to the rapid but imperfect enzymatic hydrolysis process used to obtain peptides.
Innovation Solution
A polypeptide with specific amino acid sequences (SEQ ID No.: 1 to SEQ ID No.: 4) is disclosed, which can activate insulin receptors, promote glucose active transport in cells, enhance adipocyte metabolism, and thus regulate blood glucose levels and prevent fat accumulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If enzymatic hydrolysis is used to obtain peptides rapidly, then productivity is improved, but manufacturing precision and product purity deteriorate
Solution Approach 1:
The patent segments the peptide production process into multiple controlled hydrolysis stages using different enzymes (protease, peptidase, aminopeptidase) with specific activity ratios. This segmentation allows rapid production while maintaining precision by controlling each stage's contribution to the final peptide profile, ensuring consistent quality and purity across batches.
Solution Approach 2:
The patent applies parameter changes by precisely controlling enzyme activity ratios, hydrolysis time, temperature, and pH conditions during the enzymatic hydrolysis process. These parameter optimizations enable the process to achieve both high productivity and high manufacturing precision, producing peptides with specific amino acid compositions and molecular weight distributions that meet strict quality requirements.
2Productivity
If enzymatic hydrolysis is used to obtain peptides rapidly, then productivity is improved, but quality control becomes more difficult
Solution Approach 1:
The patent implements feedback control by monitoring amino acid composition, molecular weight distribution, and hydrolysis degree during the enzymatic process. Based on real-time data, enzyme activity ratios and hydrolysis conditions are adjusted to maintain consistent peptide quality. This feedback mechanism ensures reliable quality control even during rapid production cycles.
Solution Approach 2:
The patent applies preliminary action by pre-determining optimal enzyme combinations and hydrolysis parameters based on desired peptide specifications. Standardized protocols are established beforehand, allowing rapid production to proceed with built-in quality assurance, eliminating the need for extensive quality control adjustments during production.
3Reliability
If peptides are used as nutritional supplements for blood glucose regulation and weight reduction, then health benefits are improved, but product complexity increases due to quality control requirements
Solution Approach 1:
The patent creates a universal peptide product with multi-functional health benefits, including blood glucose regulation, fat metabolism enhancement, and anti-diabetic effects. By designing peptides with specific amino acid compositions that simultaneously provide multiple health benefits, the patent simplifies the quality control process compared to producing multiple separate peptide products for different functions.
Solution Approach 2:
The patent produces composite peptide materials with controlled amino acid compositions and molecular weight distributions that integrate multiple health-promoting properties. This composite approach allows a single product to deliver comprehensive health benefits while maintaining streamlined quality control through standardized composition specifications.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptide effectively regulates blood glucose levels and enhances fat metabolism, demonstrating potential in preventing or treating metabolic syndrome, diabetes, and other related diseases.
Implementation Method 1
activate an insulin receptor, to regulate blood glucose level by encouraging cells to perform active transport of glucose
Implementation Method 2
enhance the oxidation and metabolism of adipocytes, and to thereby effectively produce the effect of regulating blood glucose level and/or preventing fat build-up
Data Source
Figure 1
AI summary
A polypeptide and a use thereof in regulating blood glucose level and/or reducing fat are disclosed. The amino acid sequence of the polypeptide includes the sequence of SEQ ID No.: 1, the sequence of SEQ ID No.: 2, the sequence of SEQ ID No.: 3, the sequence of SEQ ID No.: 4, or a sequence obtained by modifying at least one amino acid in any of the aforesaid sequences. The polypeptide disclosed herein has the physiological activity to enhance fat metabolism and regulate blood glucose level. Therefore, administering an effective amount of the polypeptide, or a composition containing the polypeptide, to an individual can effectively produce the effect of treating or preventing a disease related to blood glucose imbalance or to imbalance in fat metabolism.