Polysaccharide Extraction via HLB-SPE Purification

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Existing methods for extracting polysaccharides from complex samples like feces or fermentation broth result in crude extracts with high impurity levels, which complicates subsequent structural analysis.

Innovation Solution

A method involving sterilization and enzyme deactivation, followed by solid-liquid separation, deproteinization, purification using HLB-SPE cartridges, alcohol precipitation, methylation, glycoside residue derivatization, and finally LC-MS analysis to achieve rapid and accurate separation and identification of polysaccharides.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional ethanol precipitation or hot water extraction is used to extract polysaccharides from complex samples, then the extraction process is simple and fast, but the extracted crude polysaccharides contain a large number of impurities

Engineering Contradiction:

Improveextraction speedVSAvoidpurification degree

Core Design Contradiction:

ProductivityVSManufacturing precision

Solution Approach 1:

The extraction process is divided into multiple sequential steps: sterilization and enzyme deactivation, solid-liquid separation, deproteinization, HLB-SPE cartridge purification, and alcohol precipitation. Each step targets specific impurities (proteins, polysaccharides, other substances) to progressively purify the target polysaccharides while maintaining extraction efficiency

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The HLB-SPE cartridge serves as an intermediary purification device between deproteinization and alcohol precipitation. It selectively adsorbs polysaccharides from the deproteinized solution, allowing other impurities to pass through, thereby achieving high-purity polysaccharide extraction without compromising extraction speed

Inventive Principle:

Principle #24Intermediary (Mediator)

2Device complexity

If traditional extraction methods are used, then the process is simple, but subsequent structural analysis is complicated due to high impurity levels

Engineering Contradiction:

Improveprocess complexityVSAvoidstructural analysis accuracy

Core Design Contradiction:

Device complexityVSMeasurement precision

Solution Approach 1:

The method performs preliminary purification actions (sterilization, deproteinization, HLB-SPE purification) before structural analysis. This removes impurities that would interfere with subsequent methylation, glycoside residue derivatization, and LC-MS analysis, ensuring accurate structural characterization without requiring overly complex analysis protocols

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The HLB-SPE cartridge changes the purification parameter by utilizing hydrophilic-lipophilic balance properties to selectively retain polysaccharides while allowing other substances to elute. This parameter-based separation achieves high purity suitable for precise structural analysis

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method enables the rapid separation and purification of polysaccharides from complex samples, reducing impurities and maintaining the integrity of saccharide content, molecular weight, and monosaccharide compositions, thereby facilitating accurate structural identification.

Implementation Method 1

purifying the deproteinized solution by using a hydrophilic lipophilic balance-solid phase extraction (HLB-SPE) cartridge to obtain a purified solution

Methodology Applied

Scientific EffectHydrophilic-lipophilic balance (HLB): Amphiphiles

Implementation Method 2

subjecting the purified solution to alcohol precipitation to obtain the polysaccharide

Methodology Applied

Scientific EffectPrecipitation: Precipitation

Implementation Method 3

liquid chromatography-mass spectrometry (LC-MS) analysis

Methodology Applied

Scientific EffectLiquid chromatography: Chromatography

Implementation Method 4

liquid chromatography-mass spectrometry (LC-MS) analysis

Methodology Applied

Scientific EffectMass spectrometry:

Data Source

PatentUS20250172545A1Method for extracting and identifying polysaccharide from complex sample

Publication Date: 2025.05.29 ZHEJIANG UNIV

AI summary

A method for extracting and identifying a polysaccharide from a complex sample. The method includes: subjecting a complex sample to be treated to sterilization and enzyme deactivation to obtain a system, and subjecting the system to solid-liquid separation to obtain a sterilization and enzyme deactivation-treated solution; deproteinizing the sterilization and enzyme deactivation-treated solution to obtain a deproteinized solution; purifying the deproteinized solution by using a hydrophilic lipophilic balance-solid phase extraction (HLB-SPE) cartridge to obtain a purified solution; subjecting the purified solution to alcohol precipitation to obtain the polysaccharide; and subjecting the polysaccharide to methylation, glycoside residue derivatization and liquid chromatography-mass spectrometry (LC-MS) analysis in sequence.