Polysaccharide-Lipid Complex for Stable Decoy Drug Delivery
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Solution Overview
Problem
Current methods for producing decoys displaying specific structures like oligosaccharides or peptides face limitations due to natural biodegradation, toxicity, proteolytic activities, immunogenic properties, and production safety concerns, which restrict their application in fields like oral vaccines and drug delivery.
Innovation Solution
A polysaccharide complex is formed by direct bonding between a polysaccharide and a heterologous lipid carrier comprising ceramide-like glycolipid or fatty acid moieties, along with carbohydrate, lipopeptide, linker, or peptide moieties, creating a stable association that resists environmental conditions and allows site-specific drug delivery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If nanoparticles are used as decoys, then they can display specific structures to the environment, but they are limited by natural biodegradation, absorption, and potential toxic effects in cells or organs where they may accumulate
Solution Approach 1:
The patent employs composite materials by combining polysaccharides (natural, biodegradable polymers) with lipid carriers (ceramide-like glycolipids or fatty acids) to create a hybrid decoy structure. This composite approach leverages the biocompatibility and stability of polysaccharides while incorporating the membrane-interaction capabilities of lipids, thereby reducing toxic effects and improving cellular uptake without compromising structural integrity.
2Reliability
If microorganisms are fused with a protein composed of a peptide of interest bound to a protein-domain, then they can display the structure of interest, but they are limited by natural proteolytic activities that may affect the stability and integrity of the fusion protein
Solution Approach 1:
The patent changes the chemical parameters of the decoy system by replacing protein-based fusion constructs with polysaccharide-lipid complexes. Polysaccharides are resistant to proteolytic degradation, thereby eliminating the stability issue inherent in protein-based approaches. The structural parameters of the decoy are modified to use glycosidic linkages instead of peptide bonds, which are not susceptible to protease activity.
3Productivity
If genetically modified microorganisms are used to express the structure of interest, then they can produce decoys, but they are coupled with production and safety issues including difficulty to produce at pharmaceutical grade, ability to mutate, potential to colonize permanently, and potential to cause diseases
Solution Approach 1:
The patent extracts the decoy production process from living genetically modified microorganisms and transfers it to an in vitro chemical or physical system. By taking out the biosynthetic function from the biological system, the patent eliminates concerns about mutation, permanent colonization, and disease causation while maintaining the ability to produce decoys at pharmaceutical grade through controlled chemical synthesis or physical assembly of polysaccharide-lipid complexes.
4Ease of manufacture
If standard procedures use chemically produced nanoparticles or genetically modified microorganisms, then decoys can be produced, but the processes are complex and raise safety concerns
Solution Approach 1:
The patent changes the production approach from complex biological or chemical synthesis to a simpler physical complexation process. By adjusting parameters such as temperature, pH, and ionic strength, polysaccharides and lipid carriers can be assembled into stable decoy structures through non-covalent interactions or simple covalent bonding, eliminating the need for complex genetic modification or multi-step chemical synthesis while ensuring production safety.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polysaccharide complex is stable under physiological conditions, enabling specific binding of pathogens and toxins, and effective site-specific drug delivery, overcoming previous limitations in decoy technology.
Implementation Method 1
wherein i) and ii) are associated by direct bonding to each other
Data Source
AI summary
The present invention relates to a polysaccharide complex comprising at least one polysaccharide (P1) and ii) a heterologous lipid carrier wherein the heterologous lipid carrier comprises a) a lipid portion comprising at least one ceramide-like glycolipid moiety and/or a fatty acid moiety and b) at least one non-lipid moiety, preferably said non-lipid portion comprises at least one carbohydrate moiety, at least one lipopeptide moiety, at least one linker, at least one chemical compound, and/or at least one peptide moiety, wherein i) and ii) are associated. Further, the invention is directed to a method to prepare said polysaccharide complex.