Functionalized Polystyrene Microspheres for Room-Temperature RNA Storage
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Solution Overview
Problem
The structural instability of mRNA during storage and transportation leads to degradation, necessitating ultra-low temperature preservation, which is costly and poses safety risks, while external protective agents compromise biosafety.
Innovation Solution
A functionalized polystyrene microsphere with specific functional groups is used as an RNA room-temperature storage stabilizer, forming a three-dimensional structure with RNA molecules to stabilize them at room temperature, allowing for long-term storage without cryopreservation or harmful additives.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If ultra-low temperature preservation is used to store mRNA, then RNA stability is improved, but storage cost and safety risk increase
Solution Approach 1:
The patent introduces a functionalized polystyrene microsphere as an intermediary substance that binds to mRNA molecules. This microsphere acts as a carrier that protects mRNA from degradation without requiring ultra-low temperature storage, thereby resolving the contradiction between maintaining RNA stability and reducing storage cost/safety risk
Solution Approach 2:
The patent changes the storage parameter from temperature-based (ultra-low temperature) to a chemical-based approach (functional group interaction). By modifying the polystyrene microsphere with specific functional groups that interact with mRNA, the system achieves stability without relying on extreme temperature conditions
2Stability of the object's composition
If external protective agents are added to protect mRNA, then RNA stability is improved, but biosafety decreases
Solution Approach 1:
The functionalized polystyrene microsphere serves as a physical carrier that protects mRNA through binding interactions. This approach replaces chemical protective agents with a mechanical/physical protection mechanism, thereby maintaining RNA stability while preserving biosafety
Solution Approach 2:
The patent uses a disposable microsphere carrier that can be easily removed or degraded. This approach avoids the use of persistent chemical protective agents, allowing for temporary protection during storage and transport while maintaining biosafety through the temporary nature of the protective mechanism
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The stabilizer provides stable RNA storage at room temperature, reducing costs and enhancing biosafety, while maintaining RNA integrity and functionality.
Implementation Method 1
forming a three-dimensional structure with RNA molecules to stabilize them at room temperature
Implementation Method 2
The functionalized polystyrene microsphere has good adsorption capacity for RNA. The functional groups on the microsphere can interact with the groups in the RNA
Data Source
AI summary
An RNA room-temperature storage stabilizer, an RNA product, and a preparation method and use thereof are disclosed, belonging to the field of biotechnology. The RNA room-temperature storage stabilizer provided by the present disclosure includes a functionalized polystyrene microsphere. The RNA room-temperature storage stabilizer has good adsorption capacity for RNA. The functional groups on the RNA room-temperature storage stabilizer can interact with the groups in the RNA, which plays a role in stabilizing the structure of the RNA, thereby realizing long-term stable storage of the RNA at room temperature without the need of introducing an ultra-low temperature device and other protective reagents; and the addition of water can destroy the binding between the functionalized polystyrene microsphere and the RNA, so as to achieve complete separation of the RNA from the stabilizer. It has the advantages of low cost, simple operation and high biosafety.