Porous Particle Reagent for Rapid Biosensor Rehydration
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Solution Overview
Problem
Conventional biosensor sensor strips using solid particles require longer times to produce analytically useful output signals, often taking more than four seconds to correlate analyte concentrations, which delays analysis and may lead to inaccuracies.
Innovation Solution
A reagent composition incorporating porous particles with an average diameter of 0.05 to 10 micrometers and a void volume of at least 20% is used, allowing for rapid rehydration and enhanced redox reaction kinetics, enabling output signal correlation with analyte concentration within two seconds or less.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If solid particles are used in the reagent composition, then the structural stability is maintained, but the analysis time increases beyond four seconds
Solution Approach 1:
The patent applies porous particles with specific pore size distributions (including micropores, mesopores, and macropores) to create a reagent composition that enables rapid analyte diffusion and reaction. The porous structure provides both quick access pathways for analytes and sufficient surface area for reaction, achieving analysis within two seconds while maintaining compositional stability through the controlled pore architecture.
Solution Approach 2:
The patent uses composite porous particles combining different materials with complementary properties, such as silica-based particles with organic modifications or combinations of different pore-sized particles. This composite approach optimizes both the rapid reaction kinetics needed for fast analysis and the structural stability required for reliable operation.
2Speed
If porous particles with high void volume are used, then the rehydration speed increases, but the particle structural integrity may compromise
Solution Approach 1:
The patent specifies porous particles with void volumes of at least 20% and controlled pore size distributions that balance rehydration speed with structural integrity. The multi-scale pore structure (combining smaller micropores with larger macropores) provides rapid water penetration pathways while the particle framework maintains sufficient mechanical strength for handling and operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of porous particles in the reagent composition significantly reduces analysis time, providing accurate analyte concentration determination within two seconds, improving the speed and precision of biosensor readings.
Implementation Method 1
The particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v)
Implementation Method 2
the analyte concentration is determined from an electrical signal generated by an oxidation/reduction or redox reaction of the analyte or a species responsive to the analyte
Implementation Method 3
An oxidoreductase, such as an enzyme or similar species, may be added to the sample to enhance the electron transfer from a first species to a second species during the redox reaction
Data Source
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AI summary
A reagent composition for a biosensor sensor strip is disclosed that provides for rapid rehydration after drying. The composition includes porous particles and is preferably formed as a colloidal suspension. The rapidly rehydrating dried composition may provide analytically useful output from the sensor strip in a shorter time period than observed from dried compositions using solid particles. The output signal from the rapidly rehydrating compositions may be correlated to the analyte concentration of a sample within about two seconds. In this manner, an accurate concentration determination of an analyte concentration in a sample may be obtained in less time than from sensor strips including conventional compositions.