Porous Substrate Capture for High-Throughput Multiplex Cell Analysis
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Solution Overview
Problem
There is a lack of a practical, cost-effective, high-throughput technique for examining and detecting bio-molecular components of individual cells or viruses.
Innovation Solution
A method involving the covalent capture and detection of bio-molecular components of individual cells or viruses on a porous solid substrate, utilizing electrophoresis, convection, or centrifugation to transfer components into interstices or pores, followed by covalent binding, and subsequent detection with specific probes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods are used to examine bio-molecular components, then detection can be performed, but throughput is low and cost is high
Solution Approach 1:
The method segments the biological sample into individual cells or viruses, each captured in separate pores of the substrate. This segmentation enables parallel processing of multiple samples simultaneously, dramatically increasing throughput while maintaining cost-effectiveness through standardized protocols
Solution Approach 2:
The invention uses a porous solid substrate where each pore acts as an independent capture site for single cells or viruses. The porous structure enables high-throughput analysis by allowing simultaneous processing of numerous samples in parallel, while the standardized pore architecture keeps manufacturing costs low
2Measurement precision
If individual cell analysis is performed, then detailed bio-molecular information is obtained, but the process becomes complex and low-throughput
Solution Approach 1:
By segmenting samples into individual cells captured in separate pores, the method simplifies the analysis process while maintaining high measurement precision. Each pore provides an isolated environment for accurate bio-molecular detection without cross-contamination, and the standardized workflow reduces operational complexity
Solution Approach 2:
The porous substrate serves multiple functions: it captures single cells, enables lysis, facilitates bio-molecular transfer, and supports detection. This multi-functionality reduces process complexity by consolidating multiple steps into a single platform while maintaining high detection accuracy for various bio-molecular components
3Productivity
If high-throughput technique is implemented, then productivity increases, but cost and complexity increase
Solution Approach 1:
The porous substrate provides a scalable platform for high-throughput analysis by enabling parallel processing of numerous samples in individual pores. The standardized pore structure allows for easy replication and scaling without proportionally increasing complexity, as the same basic mechanism operates across all pores simultaneously
Solution Approach 2:
The method merges multiple functions (capture, lysis, transfer, and detection) into a single integrated porous substrate system. This consolidation enables high-throughput processing by eliminating the need for separate complex equipment for each function, thereby increasing productivity without proportionally increasing overall system complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high-throughput examination and detection of multiple bio-molecular components from individual cells or viruses, allowing for repeated probing and quantification.
Implementation Method 1
transferring the bio-molecular components of the biological sample is accomplished through electrophoresis
Implementation Method 2
transferring the bio-molecular components of the biological sample is accomplished through convection
Implementation Method 3
transferring the bio-molecular components of the biological sample is accomplished through centrifugation
Implementation Method 4
affixing (e.g., via covalent binding) the bio-molecular components of the biological sample to the interstices or pores of the solid substrate
Data Source
AI summary
Methods of transferring bio-molecular components of individual cells in a biological sample to a solid porous substate, as well as methods of examining or detecting one or more bio-molecular components of individual cells in a biological sample. The method transferring including contacting the biological sample to a first side of the porous solid substrate having a plurality of interstices or pores extending contiguously from the first side to a second side, transferring and affixing the bio-molecular components of the biological sample to the interstices or pores of the solid substrate, and the method of examining or detecting includes detecting one or more of the bio-molecular components of the biological sample transferred to the solid porous substrate.